Elongation factor 1 alpha genes of the red alga Porphyra purpurea include a novel, developmentally specialized variant

被引:18
作者
Liu, QY
Baldauf, SL
Reith, ME
机构
[1] NATL RES COUNCIL CANADA,INST BIOL SCI,OTTAWA,ON K1A 0R6,CANADA
[2] NATL RES COUNCIL CANADA,INST MARINE BIOSCI,OTTAWA,ON K1A 0R6,CANADA
[3] DALHOUSIE UNIV,DEPT BIOL,HALIFAX,NS B3H 4J1,CANADA
关键词
developmental regulation; elongation factor; evolution; gene expression; rhodophyte; sporophyte;
D O I
10.1007/BF00020608
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The life cycle of the red alga Porphyra purpurea alternates between two morphologically distinct phases: a shell-boring, filamentous sporophyte and a free-living, foliose gametophyte. From a subtracted cDNA library enriched for sporophyte-specific sequences, we isolated a cDNA encoding an unusual elongation factor 1 alpha (EF-1 alpha) that is expressed only in the sporophyte. A second EF-1 alpha gene that is expressed equally in the sporophyte and the gametophyte was isolated from a genomic library. These are the only EF-1 alpha genes detectable in P. purpurea. The constitutively expressed gene encodes an EF-la very similar to those of most eukaryotes. However, the sporophyte-specific EF-1 alpha is one of the most divergent yet described, with nine insertions or deletions ranging in size from 1 to 26 amino acids. This is the first report of a developmental stage-specific EF-lx outside of the animal kingdom and suggests a fundamental role for EF-1 alpha in the developmental process.
引用
收藏
页码:77 / 85
页数:9
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