Ultrasensitive and Fast Bottom-up Analysis of Femtogram Amounts of Complex Proteome Digests

被引：175
作者：
Sun, Liangliang [1 ]
Zhu, Guijie [1 ]
Zhao, Yimeng [1 ]
Yan, Xiaojing [1 ]
Mou, Si [1 ]
Dovichi, Norman J. [1 ]
机构：
[1] Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 51期
基金：
美国国家卫生研究院;
关键词：
electrophoresis; electrospray; mass spectrometry; proteomics; ultrasensitive analysis; CAPILLARY-ZONE-ELECTROPHORESIS; TANDEM MASS-SPECTROMETRY; ELECTROSPRAY INTERFACE; PEPTIDE ANALYSIS; HIGH-EFFICIENCY; SEPARATION; MS; SENSITIVITY; COLUMNS; MS/MS;
D O I：
10.1002/anie.201308139
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Femtogram proteomics: An ultrasensitive capillary zone electrophoresis-mass spectrometry system that is based on an improved nanospray interface has been developed. This system is used for the analysis of picogram to femtogram amounts of E. coli digests; for example, over 100 proteins were identified from 16 pg digests by tandem mass spectrometry. AMTs=accurate mass and time tags. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：13661 / 13664
页数：4
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