Messenger RNA profiling: A novel method for body fluid identification by Real-Time PCR

被引：128
作者：
Nussbaumer, C [1 ]
Gharehbaghi-Schnell, E [1 ]
Korschineck, I [1 ]
机构：
[1] Ingenetix Ltd, A-1110 Vienna, Austria
来源：
FORENSIC SCIENCE INTERNATIONAL | 2006年 / 157卷 / 2-3期
基金：
奥地利科学基金会;
关键词：
mRNA-profiling; real-time PCR; body fluids; gene expression;
D O I：
10.1016/j.forsciint.2005.10.009
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
Conventional methods for the identification of different body fluids like blood, semen and saliva from biological stains involve immunological or enzymatic detection of certain proteins. In this study, we investigated potential RNA markers with the aim of developing Real-Time polymerase chain reaction (PCR) based methods to allow differentiation between several body fluids. Total RNA samples from artificially stained swabs and from various pieces of evidence from case work were extracted, amplified and analyzed with several RNA markers. Three assays detecting the body fluids of interest were selected: hemoglobinalpha locus 1 (HBA), kallikrein 3 (KLK) and mucin 4 (MUC). With this approach, we demonstrate that specific Real-Time PCR assays are useful in identifying the source of the biological stain. Furthermore, RNA profiling of various body fluids was even possible on samples stored over a long period of time at ambient temperature. The stability and sensitivity of the applied method outlines a novel application for Real-Time PCR within the forensic field. (C) 2005 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：181 / 186
页数：6
相关论文
共 15 条
  • [1] A method for determining the age of a bloodstain
    Anderson, S
    Howard, B
    Hobbs, GR
    Bishop, CP
    [J]. FORENSIC SCIENCE INTERNATIONAL, 2005, 148 (01) : 37 - 45
  • [2] Quantification of RNA degradation by semi-quantitative duplex and competitive RT-PCR: a possible indicator of the age of bloodstains?
    Bauer, M
    Polzin, S
    Patzelt, D
    [J]. FORENSIC SCIENCE INTERNATIONAL, 2003, 138 (1-3) : 94 - 103
  • [3] Bauer M, 2002, J FORENSIC SCI, V47, P1278
  • [4] The human transcriptome map:: Clustering of highly expressed genes in chromosomal domains
    Caron, H
    van Schaik, B
    van der Mee, M
    Baas, F
    Riggins, G
    van Sluis, P
    Hermus, MC
    van Asperen, R
    Boon, K
    Voûte, PA
    Heisterkamp, S
    van Kampen, A
    Versteeg, R
    [J]. SCIENCE, 2001, 291 (5507) : 1289 - +
  • [5] Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) for the measurement of prostate-specific antigen mRNA in the peripheral blood of patients with prostate carcinoma using the TaqMan™ detection system
    Gelmini, S
    Tricarico, C
    Vona, G
    Livi, L
    Della Melina, A
    Semi, S
    Cellai, E
    Magrini, S
    Villari, D
    Carini, M
    Serio, M
    Forti, G
    Pazzagli, M
    Orlando, C
    [J]. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2001, 39 (05) : 385 - 391
  • [6] Green RL, 2005, J FORENSIC SCI, V50, P809
  • [7] The use of real-time PCR analysis in a gene expression study of Alzheimer's disease post-mortem brains
    Gutala, RV
    Reddy, PH
    [J]. JOURNAL OF NEUROSCIENCE METHODS, 2004, 132 (01) : 101 - 107
  • [8] Degradation profile of mRNA in a dead rat body: basic semi-quantification study
    Inoue, H
    Kimura, A
    Tuji, T
    [J]. FORENSIC SCIENCE INTERNATIONAL, 2002, 130 (2-3) : 127 - 132
  • [9] DETECTION OF HUMAN HEMOGLOBIN A (HBA) AND HUMAN HEMOGLOBIN F (HBF) IN BIOLOGICAL STAINS BY MICROTITER LATEX AGGLUTINATION-INHIBITION TEST
    ITOH, Y
    MATSUZAWA, S
    [J]. FORENSIC SCIENCE INTERNATIONAL, 1990, 47 (01) : 79 - 89
  • [10] Messenger RNA profiling: a prototype method to supplant conventional methods for body fluid identification
    Juusola, J
    Ballantyne, J
    [J]. FORENSIC SCIENCE INTERNATIONAL, 2003, 135 (02) : 85 - 96
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