Investigation of Deletion Variation and Methylation Patterns in the 5′ LTR of Porcine Endogenous Retroviruses

被引：3

作者：

Jung, K. C. ^{[1
]}

Simond, D. M. ^{[1
]}

Moran, C. ^{[1
]}

Hawthorne, W. J. ^{[1
]}

Jeon, J. T. ^{[1
]}

Jin, D. I. ^{[1
]}

Lee, J. H. ^{[1
]}

机构：

[1] Chungnam Natl Univ, Div Anim Sci & Resources, Res Ctr Transgen Cloned Pigs, Taejon 305764, South Korea

来源：

ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | 2008年 / 21卷 / 11期

关键词：

Pigs; Long Terminal Repeat (LTR); Porcine Endogenous Retroviruses (PERVs); Xenotransplantation;

D O I：

10.5713/ajas.2008.80065

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The xenotransplantation of pig organs and cells can be related with a risk of transmission of infectious diseases to human. Previous findings indicate that the regulatory region of PERV for retroviral transcription, replication and integration into the cellular DNA is located on the 5' Long Terminal Repeat (LTR). The objective of this study is the investigation of methylation and deletion status of the PERV 5' LTR region which can be used for regulating PERV expression. We compared the sequences of genomic DNA and bisulfite-treated genomic DNA from PK-15 cells expressing PERV to observe the methylation status of the 5 LTR. Our results showed that the CpG sites of U3 were methylated and methylation was inconsistent in the R and U5 regions. Also, variable numbers of 18 bp repeats and 21 bp repeats were detected on 5' LTR by sequencing analysis. The consistent U3 methylation might be indicative of host suppression of expression of the retroviruses.

引用

页码：1572 / 1575

页数：4

共 13 条

[1] INACTIVATION OF THE HIV LTR BY DNA CPG METHYLATION - EVIDENCE FOR A ROLE IN LATENCY [J].