Overexpression of LncRNA AFAP1-AS1 predicts poor prognosis and promotes cells proliferation and invasion in gallbladder cancer

被引：62
作者：
Ma, Fei [1 ]
Wang, Shou-Hua [2 ]
Cai, Qiang [2 ]
Zhang, Ming-Di [2 ]
Yang, Yong [2 ]
Ding, Jun [3 ]
机构：
[1] Shanghai Jiao Tong Univ, Xinhua Hosp, Dept Oncol, Sch Med, Shanghai 200092, Peoples R China
[2] Shanghai Jiao Tong Univ, Xinhua Hosp, Dept Gen Surg, Sch Med, Shanghai 200000, Peoples R China
[3] Shanghai Univ TCM, Dept Biliary & Pancreat Surg, Shanghai Shuguang Hosp, 528 Zhangheng Rd, Shanghai 201203, Peoples R China
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2016年 / 84卷
关键词：
Gallbladder cancer; lncRNA AFAP1-AS1; Epithelial-mesenchymal transition; Tumor invasion; LONG NONCODING RNA; EPITHELIAL-MESENCHYMAL TRANSITIONS; CARCINOMA; ADENOCARCINOMA; EXPRESSION;
D O I：
10.1016/j.biopha.2016.10.064
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background: Long non-coding RNA actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1) has been elucidated to be associated with some kinds of human cancers. However, whether lncRNA AFAP1-AS1 implicates in tumor development of gallbladder cancer (GBC) remains largely unknown. This study aims to elucidate the tumorigenic role and regulatory function of lncRNA AFAP1-AS1 in gallbladder cancer. Methods: We analyzed lncRNA AFAP1-AS1 expression by quantitative real time PCR (qRT-PCR) in 40 gallbladder cancer tissue and adjacent normal tissues, survival plots were generated by Kaplan-Meier analysis and the log-rank test. The expression levels of transcription factor Twist1 and epithelial-to mesenchymal transition (EMT) makers (E-cadherin and Vimentin) were detected by quantitative real time PCR and western blotting analysis after knockdown of lncRNA AFAP1-AS1. Results: The expression levels of lncRNA AFAP1-AS1 were significantly elevated in GBC tissues and GBC cell lines. In addition, the expression level of lncRNA AFAP1-AS1 was significantly associated with tumor sizes and the higher expression of lncRNA AFAP1-AS1 was correlated with poor prognosis in GBC patients. Knockdown of LncRNA AFAP1-AS1 suppressed cell growth and invasion in NOZ and GBC-SD cells. Furthermore, we found that knockdown of LncRNA AFAP1-AS1 in GBC cells inhibited EMT by down-regulating the transcription factor Twist1 and Vimentin and up-regulated the E-cadherin. Conclusions: Our results suggested lncRNA AFAP1-AS1 was correlated with poor prognosis in GBC patients and lncRNA AFAP1-AS1 might be novel therapeutic target in gallbladder cancer. (C) 2016 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：1249 / 1255
页数：7
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