Bacterial Communities Associated with Production Facilities of Two Newly Drilled Thermogenic Natural Gas Wells in the Barnett Shale (Texas, USA)

被引:71
作者
Davis, James P. [1 ]
Struchtemeyer, Christopher G. [1 ]
Elshahed, Mostafa S. [1 ]
机构
[1] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74074 USA
基金
美国国家科学基金会;
关键词
16S RIBOSOMAL-RNA; FORT-WORTH BASIN; SP-NOV; OIL-FIELD; MICROBIAL DIVERSITY; GEN-NOV; REDUCING BACTERIA; PIPELINES; WATER; ENVIRONMENTS;
D O I
10.1007/s00248-012-0073-3
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
We monitored the bacterial communities in the gas-water separator and water storage tank of two newly drilled natural gas wells in the Barnett Shale in north central Texas, using a 16S rRNA gene pyrosequencing approach over a period of 6 months. Overall, the communities were composed mainly of moderately halophilic and halotolerant members of the phyla Firmicutes and Proteobacteria (classes I'eta-, Gamma-, and Epsilonproteobacteria) in both wells at all sampling times and locations. Many of the observed lineages were encountered in prior investigations of microbial communities from various fossil fluid formations and production facilities. In all of the samples, multiple H2S-producing lineages were encountered; belonging to the sulfate- and sulfur-reducing class Deltaproteobacteria, order Clostridiales, and phylum Synergistetes, as well as the thiosulfate-reducing order Halanaerobiales. The bacterial communities from the separator and tank samples bore little resemblance to the bacterial communities in the drilling mud and hydraulic-fracture waters that were used to drill these wells, suggesting the in situ development of the unique bacterial communities in such well components was in response to the prevalent geochemical conditions present. Conversely, comparison of the bacterial communities on temporal and spatial scales suggested the establishment of a core microbial community in each sampled location. The results provide the first overview of bacterial dynamics and colonization patterns in newly drilled, thermogenic natural gas wells and highlights patterns of spatial and temporal variability observed in bacterial communities in natural gas production facilities.
引用
收藏
页码:942 / 954
页数:13
相关论文
共 49 条
[1]  
Alain K, 2002, INT J SYST EVOL MICR, V52, P1621, DOI [10.1099/ijs.0.02142-0, 10.1099/00207713-52-5-1621]
[2]   Chromohalobacter salexigens sp nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174 [J].
Arahal, DR ;
García, MT ;
Vargas, C ;
Cánovas, D ;
Nieto, JJ ;
Ventosa, A .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :1457-1462
[3]  
Bowker K.A., 2003, West Texas Geological Society Bulletin, V42, P4
[4]   Barnett Shale gas production, Fort Worth Basin: Issues and discussion [J].
Bowker, Kent A. .
AAPG BULLETIN, 2007, 91 (04) :523-533
[5]  
CHAO A, 1993, BIOMETRIKA, V80, P193, DOI 10.1093/biomet/80.1.193
[6]   ESTIMATING THE NUMBER OF CLASSES VIA SAMPLE COVERAGE [J].
CHAO, A ;
LEE, SM .
JOURNAL OF THE AMERICAN STATISTICAL ASSOCIATION, 1992, 87 (417) :210-217
[7]   SPECTROPHOTOMETRIC DETERMINATION OF HYDROGEN SULFIDE IN NATURAL WATERS [J].
CLINE, JD .
LIMNOLOGY AND OCEANOGRAPHY, 1969, 14 (03) :454-&
[8]   Arcobacter mytili sp nov., an indoxyl acetate-hydrolysis-negative bacterium isolated from mussels [J].
Collado, Luis ;
Cleenwerck, Ilse ;
Van Trappen, Stefanie ;
De Vos, Paul ;
Jose Figueras, Maria .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2009, 59 :1391-1396
[9]   SULFATE-REDUCING BACTERIA AND THEIR ACTIVITIES IN OIL PRODUCTION [J].
CORDRUWISCH, R ;
KLEINITZ, W ;
WIDDEL, F .
JOURNAL OF PETROLEUM TECHNOLOGY, 1987, 39 (01) :97-106
[10]   Microbial community structure analysis of produced water from a high-temperature North Sea oil-field [J].
Dahle, Hakon ;
Garshol, Frydis ;
Madsen, Marit ;
Birkeland, Nils-Kare .
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2008, 93 (1-2) :37-49