Heteropolymorphism of mitochondrial NADH dehydrogenase subunit 3 gene for the population analysis of chum salmon, Oncorhynchus keta

被引：0
作者：
Yoon, M. [1 ]
Choi, Y. S. [1 ]
Jin, H. J. [1 ]
Sohn, Y. C. [1 ]
Lee, S. K. [2 ]
Jin, D. H. [1 ]
机构：
[1] Kangnung Natl Univ, Fac Marine Biosci & Technol, Kangnung 210702, South Korea
[2] Kangnung Natl Univ, Coll Dent, Kangnung 210702, South Korea
来源：
JOURNAL OF ENVIRONMENTAL BIOLOGY | 2008年 / 29卷 / 04期
关键词：
chum salmon; mitochondrial NADH dehydrogenase gene; SNP;
D O I：
暂无
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Mitochondrial DNAs (mtDNAs) has been frequently used as genetic markers for the population genetic studies. In this study, we used chum salmon (Oncorhynchus keta) from Korea, Japan and America, and compared their mitochondrial NADH dehydrogenase subunit 3 (ND3) genes by DNA sequence analysis. Sequence variation was studied in the ND3 among total 11 individuals from three populations. The ND3 gene was amplified by PCR targeting parts of cytochrome oxidase III gene (COIII) and NADH dehydrogenase subunit 4L gene (ND4L). ND3 gene sequence, encoded 752 bps, presented some genetic variation in the chum salmon populations. The observed nucleotide variations inferred the distinct genetic differentiation of American salmons from Korean and Japanese chum salmons. Six sites of single nucleotide polymorphism (SNP) were explored in the ND3 locus. Denaturing gradient gel electrophoresis analysis also showed a clear heterogenous band in American salmons compared to Asian salmons.
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页码：567 / 570
页数：4
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