Characterisation and immunostimulatory activity of an α-(1→6)-D-glucan from the cultured Armillariella tabescens mycelia

被引：78

作者：

Luo, Xia ^{[1
]}

Xu, Xiaoyan ^{[2
]}

Yu, Mengyao ^{[1
,2
]}

Yang, Zhirong ^{[1
]}

Zheng, Linyong ^{[1
,3
]}

机构：

[1] Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China

[2] Sichuan Inst Chinese Mat Med, Chengdu 610041, Peoples R China

[3] Sichuan Acad Agr Sci, Soil & Fertilizer Inst, Chengdu 610066, Peoples R China

来源：

FOOD CHEMISTRY | 2008年 / 111卷 / 02期

关键词：

Armillariella tabescens; polysaccharide; macrophage; nitric oxide; cytokines;

D O I：

10.1016/j.foodchem.2008.03.076

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

IPS-B2, an intracellular polysaccharide with anti-tumor effect, was isolated from cultured Armillariella tabescens mycelia by hot water extraction, anion-exchange and gel chromatography. Based on the results of paper chromatography (PC), gas chromatography (GC), infra-red (IR) spectroscopy and C-13 NMR, IPS-B2 was characterized as an alpha-(1 -> 6)-D-glucan with a molecular weight of 49.5 kDa. The effects of IPS-B2 on murine peritoneal macrophages were further investigated. The results demonstrated that IPS-B2 induced nitric oxide (NO) and cytokines (TNF-alpha, IL-1 beta and IL-6) production in macrophages. The outcomes of real-time reverse transcription-polymerase chain reaction (RT-PCR) proved that the transcribing level of inducible NO synthase (iNOS), TNF-alpha, IL-1 beta and IL-6 mRNA in the peritoneal macrophages have been augmented by IPS-B2. These data suggest that the anti-tumor activity of the polysaccharide from A. tabescens may due to activation of macrophage. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：357 / 363

页数：7

共 29 条

[1]

ALUM N, 1985, PLANTA MED, V50, P308

[2] Chemical modifications of the (1→3)-α-D-glucan from spores of Ganoderma lucidum and investigation of their physicochemical properties and immunological activity [J].

Bao, XF ;

Duan, JY ;

Fang, XY ;

Fang, JN .

CARBOHYDRATE RESEARCH, 2001, 336 (02) :127-140

[3] INFRA-RED SPECTRA OF CARBOHYDRATES .1. SOME DERIVATIVES OF D-GLUCOPYRANOSE [J].

BARKER, SA ;

BOURNE, EJ ;

STACEY, M ;

WHIFFEN, DH .

JOURNAL OF THE CHEMICAL SOCIETY, 1954, (JAN) :171-176

[4] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].

DUBOIS, M ;

GILLES, KA ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356

[5] THERAPY OF CANCER METASTASIS BY SYSTEMIC ACTIVATION OF MACROPHAGES - FROM THE BENCH TO THE CLINIC [J].

FIDLER, IJ ;

KLEINERMAN, ES .

RESEARCH IN IMMUNOLOGY, 1993, 144 (04) :284-287

[6] COMPARISON OF INVITRO-CELL CYTO-TOXIC ASSAYS FOR TUMOR NECROSIS FACTOR [J].

FLICK, DA ;

GIFFORD, GE .

JOURNAL OF IMMUNOLOGICAL METHODS, 1984, 68 (1-2) :167-175

[7]

[高婷 Gao Ting], 2005, [四川大学学报. 自然科学版, Journal of Sichuan University.Natural Science edition], V42, P395

[8] DIRECT EVIDENCE FOR TUMOR NECROSIS FACTOR-INDUCED MITOCHONDRIAL REACTIVE OXYGEN INTERMEDIATES AND THEIR INVOLVEMENT IN CYTOTOXICITY [J].

GOOSSENS, V ;

GROOTEN, J ;

DEVOS, K ;

FIERS, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (18) :8115-8119

[9]

KELLER R, 1990, CANCER RES, V50, P1421

[10]

KIHO T, 1992, CHEM PHARM BULL, V40, P2110, DOI 10.1248/cpb.40.2110

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