Optical tweezing electrophoresis of single biotinylated colloidal particles for avidin concentration measurement

被引:6
作者
Brans, Toon [1 ,2 ]
Strubbe, Filip [1 ,2 ]
Schreuer, Caspar [1 ,2 ]
Neyts, Kristiaan [1 ,2 ]
Beunis, Filip [1 ,2 ]
机构
[1] Univ Ghent, Elect & Informat Syst, B-9000 Ghent, Belgium
[2] Univ Ghent, Ctr Nano & Biophoton NB Photon, B-9000 Ghent, Belgium
关键词
MICROELECTROPHORESIS; TWEEZERS; SURFACE;
D O I
10.1063/1.4922039
中图分类号
O59 [应用物理学];
学科分类号
摘要
We present a novel approach for label-free concentration measurement of a specific protein in a solution. The technique combines optical tweezers and microelectrophoresis to establish the electrophoretic mobility of a single microparticle suspended in the solution. From this mobility measurement, the amount of adsorbed protein on the particle is derived. Using this method, we determine the concentration of avidin in a buffer solution. After calibration of the setup, which accounts for electro-osmotic flow in the measurement device, the mobilities of both bare and biotinylated microspheres are measured as a function of the avidin concentration in the mixture. Two types of surface adsorption are identified: the biotinylated particles show specific adsorption, resulting from the binding of avidin molecules with biotin, at low avidin concentrations (below 0.04 mu g/ml) while at concentrations of several lg/ml non-specific on both types of particles is observed. These two adsorption mechanisms are incorporated in a theoretical model describing the relation between the measured mobility and the avidin concentration in the mixture. This model describes the electrophoretic mobility of these particles accurately over four orders of magnitude of the avidin concentration. (C) 2015 AIP Publishing LLC.
引用
收藏
页数:6
相关论文
共 21 条
  • [1] Biomarkers and surrogate endpoints: Preferred definitions and conceptual framework
    Atkinson, AJ
    Colburn, WA
    DeGruttola, VG
    DeMets, DL
    Downing, GJ
    Hoth, DF
    Oates, JA
    Peck, CC
    Schooley, RT
    Spilker, BA
    Woodcock, J
    Zeger, SL
    [J]. CLINICAL PHARMACOLOGY & THERAPEUTICS, 2001, 69 (03) : 89 - 95
  • [2] Power spectrum analysis for optical tweezers
    Berg-Sorensen, K
    Flyvbjerg, H
    [J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 2004, 75 (03) : 594 - 612
  • [3] Beyond Millikan: The Dynamics of Charging Events on Individual Colloidal Particles
    Beunis, Filip
    Strubbe, Filip
    Neyts, Kristiaan
    Petrov, Dmitri
    [J]. PHYSICAL REVIEW LETTERS, 2012, 108 (01)
  • [4] Optical trapping and binding
    Bowman, Richard W.
    Padgett, Miles J.
    [J]. REPORTS ON PROGRESS IN PHYSICS, 2013, 76 (02)
  • [5] SOI optical microring resonator with poly(ethylene glycol) polymer brush for label-free biosensor applications
    De Vos, Katrien
    Girones, Jordi
    Popelka, Stepan
    Schacht, Etienne
    Baets, Roel
    Bienstman, Peter
    [J]. BIOSENSORS & BIOELECTRONICS, 2009, 24 (08) : 2528 - 2533
  • [6] Microelectrophoresis of a bilayer-coated silica bead in an optical trap:: Application to enzymology
    Galneder, R
    Kahl, V
    Arbuzova, A
    Rebecchi, M
    Rädler, JO
    McLaughlin, S
    [J]. BIOPHYSICAL JOURNAL, 2001, 80 (05) : 2298 - 2309
  • [7] Electrophoresis of spherical polymer-coated colloidal particles
    Hill, RJ
    Saville, DA
    Russel, WB
    [J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2003, 258 (01) : 56 - 74
  • [8] Camera-based three-dimensional real-time particle tracking at kHz rates and Angstrom accuracy
    Huhle, Alexander
    Klaue, Daniel
    Brutzer, Hergen
    Daldrop, Peter
    Joo, Sihwa
    Otto, Oliver
    Keyser, Ulrich F.
    Seidel, Ralf
    [J]. NATURE COMMUNICATIONS, 2015, 6
  • [9] Label-free biological and chemical sensors
    Hunt, Heather K.
    Armani, Andrea M.
    [J]. NANOSCALE, 2010, 2 (09) : 1544 - 1559
  • [10] Development of AC microelectrophoresis for rapid protein affinity evaluation
    Okada, Tomoko
    Yamamoto, Yuji
    Shibuya, Tsubasa
    Kang, Hyen-Wook
    Miyachi, Hirotaka
    Karube, Isao
    Muramatsu, Hiroshi
    Kim, Jong Min
    [J]. BIOCHEMICAL ENGINEERING JOURNAL, 2008, 41 (01) : 17 - 23