Dynamics of CO Rebinding to Protoheme in Viscous Solutions

被引：0

作者：

Lee, Taegon ^{[1
,2
]}

Park, Jaeheung ^{[1
,2
]}

Kim, Jooyoung ^{[1
,2
]}

Joo, Sangwoo ^{[3
]}

Lim, Manho ^{[1
,2
]}

机构：

[1] Pusan Natl Univ, Dept Chem, Pusan 609735, South Korea

[2] Pusan Natl Univ, Chem Inst Funct Mat, Pusan 609735, South Korea

[3] Soongsil Univ, Dept Chem, Seoul 156743, South Korea

来源：

BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2009年 / 30卷 / 01期

关键词：

CO Rebinding; Protoheme; Fs vibrational spectroscopy; Viscous solution; LIGAND-BINDING KINETICS; CARBON-MONOXIDE; INFRARED-SPECTROSCOPY; GEMINATE RECOMBINATION; HEME-PROTEINS; ORGANIC-SOLVENTS; OPTICAL-SPECTRA; HUMAN MYOGLOBIN; HEMOGLOBIN; VISCOSITY;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present the geminate rebinding kinetics measurements of CO to 2-methylimidazole (2-MeIm) bound ferrous protoporphyrin-IX (FePPIX) in alkaline glycerol/water mixtures at 293 K after photolysis. The kinetics was probed by monitoring the CO stretching mode using femtosecond vibrational spectroscopy. When 2-MeIm is used in excess, heme dimers that typically form in low viscosity solutions disappear as the viscosity of the solvent increases. Heme aggregates formed in low viscosity solutions turn monomeric as more 2-Meim is added, suggesting that 6-coordinated heme, including a strong proximal histidine tends to be in the monomeric form. The vibrational band of CO in the 2-MeIM-FePPIX-CO is well described by a single Gaussian function centered at 1958 cm(-1) and 28 cm(-1) full width at half maximum. The efficiency and rate of the geminate rebinding of CO to the heme increase with viscosity of the solvent, suggesting that retention of the dissociated CO near the heme, for a longer period by the viscous solvent media, accelerates rebinding.

引用

页码：177 / 182

页数：6

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