Effects of Rutin on Wound Healing in Hyperglycemic Rats

被引：74

作者：

Chen, Li-You ^{[1
,2
]}

Huang, Chien-Ning ^{[3
,4
]}

Liao, Chih-Kai ^{[1
,2
]}

Chang, Hung-Ming ^{[5
]}

Kuan, Yu-Hsiang ^{[6
,7
]}

Tseng, To-Jung ^{[1
,2
]}

Yen, Kai-Jung ^{[1
]}

Yang, Kai-Lin ^{[1
]}

Lin, Hsing-Chun ^{[8
,9
]}

机构：

[1] Chung Shan Med Univ, Coll Med, Sch Med, Dept Anat, Taichung 40201, Taiwan

[2] Chung Shan Med Univ Hosp, Dept Med Educ, Taichung 40201, Taiwan

[3] Chung Shan Med Univ Hosp, Div Endocrinol & Metab, Dept Internal Med, Taichung 40201, Taiwan

[4] Chung Shan Med Univ, Coll Med, Inst Med, Taichung 40201, Taiwan

[5] Taipei Med Univ, Coll Med, Sch Med, Dept Anat & Cell Biol, Taipei 40201, Taiwan

[6] Chung Shan Med Univ, Sch Med, Dept Pharmacol, Taichung 40201, Taiwan

[7] Chung Shan Med Univ Hosp, Dept Pharm, Taichung 40201, Taiwan

[8] Chung Shan Med Univ, Dept Nutr, Taichung 40201, Taiwan

[9] Chung Shan Med Univ Hosp, Dept Nutr, Taichung 40201, Taiwan

来源：

ANTIOXIDANTS | 2020年 / 9卷 / 11期

关键词：

rutin; hyperglycemia; wound healing; antioxidant; anti-inflammatory; INDUCED OXIDATIVE STRESS; GLUCOSE-HOMEOSTASIS; DIABETIC-RATS; STREPTOZOTOCIN; INFLAMMATION; DYSLIPIDEMIA; MARKER; IMPACT; SEEDS;

D O I：

10.3390/antiox9111122

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Long-term poor glycemic control negatively affects macrovascular and microvascular diseases, as well as wound restoration. Buckwheat is a good source of rutin (quercetin-3-O-rutoside) and has benefits in regulating blood sugar. This study was to evaluate the antioxidant and anti-inflammatory effects of rutin on wound healing in streptozotocin-induced hyperglycemic rats. Eighteen male Wistar rats were randomly divided into three groups: normal (NDM), hyperglycemic (DM), and hyperglycemic with rutin (DMR). After induction of hyperglycemia for 2 days, a 15 x 15 mm wound was induced on the back of each rat. Intraperitoneal injection of rutin significantly ameliorated diabetes-induced body weight loss and improved metabolic dysfunctions of hyperglycemic rats. Based on appearance and histopathological staining, rutin promotes wound healing and inhibits production of inflammatory cells. The immunoblotting data indicated that rutin promotes production of antioxidant enzymes induced by nuclear factor erythroid 2-related factor 2 (NRF2), inhibits the expression of matrix metalloproteinases (MMPs) regulated by NF-kappa B, and decreases the expression of vascular endothelial growth factor (VEGF). It also promotes the expression of neurogenic-related protein (UCH-L1). The aforementioned results indicated that rutin reduces oxidative stress and inflammatory response in hyperglycemic rats, promoting wound healing and subsequently reducing the risk of wound ulcers.

引用

页码：1 / 13

页数：13

共 46 条

[1]

[Anonymous], 2019, IDF Diabetes Atlas 2019, VNinth

[2]

Ansel J C, 2001, Exp Dermatol, V10, P352

[3]

Aruna Ravikumar, 2014, J Complement Integr Med, V11, P9, DOI 10.1515/jcim-2013-0023

[4] Rutin, quercetin, and free amino acid analysis in buckwheat (Fagopyrum) seeds from different locations [J].

Bai, C. Z. ;

Feng, M. L. ;

Hao, X. L. ;

Zhong, Q. M. ;

Tong, L. G. ;

Wang, Z. H. .

GENETICS AND MOLECULAR RESEARCH, 2015, 14 (04) :19040-19048

[5] Lupeol, a Dietary Triterpene, Enhances Wound Healing in Streptozotocin-Induced Hyperglycemic Rats with Modulatory Effects on Inflammation, Oxidative Stress, and Angiogenesis [J].

Beserra, Fernando Pereira ;

Vieira, Ana Julia ;

Sergio Gushiken, Lucas Fernando ;

de Souza, Eduardo Oliveira ;

Hussni, Maria Fernanda ;

Hussni, Carlos Alberto ;

Nobrega, Rafael Henrique ;

Monteiro Martinez, Emanuel Ricardo ;

Jackson, Christopher John ;

de Azevedo Maia, Gabriela Lemos ;

Rozza, Ariane Leite ;

Pellizzon, Claudia Helena .

OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2019, 2019

[6] Protein gene product 9.5 (PGP 9.5) is not a specific marker of neural and nerve sheath tumors: An immunohistochemical study of 95 mesenchymal neoplasms [J].

Campbell, LK ;

Thomas, JR ;

Lamps, LW ;

Smoller, BR ;

Folpe, AL .

MODERN PATHOLOGY, 2003, 16 (10) :963-969

[7] Targeting Oxidative Stress and Mitochondrial Dysfunction in the Treatment of Impaired Wound Healing: A Systematic Review [J].

Cano Sanchez, Mariola ;

Lancel, Steve ;

Boulanger, Eric ;

Neviere, Remi .

ANTIOXIDANTS, 2018, 7 (08)

[8] Restructuring of the extracellular matrix in diabetic wounds and healing: A perspective [J].

Chang, Mayland .

PHARMACOLOGICAL RESEARCH, 2016, 107 :243-248

[9]

Chawla Aastha, 2016, Indian J Endocrinol Metab, V20, P546, DOI 10.4103/2230-8210.183480

[10]

CHENG KY, 2018, SCI REP UK, V0008

← 1 2 3 4 5 →