Laccase-initiated reaction between phenolic acids and chitosan

被引:51
作者
Bozic, Mojca [1 ]
Strancar, Janez [2 ]
Kokol, Vanja [1 ]
机构
[1] Univ Maribor, Inst Engn Mat & Design, SI-2000 Maribor, Slovenia
[2] Jozef Stefan Inst, Biophys Lab, SI-1000 Ljubljana, Slovenia
关键词
Laccase; Chitosan; Phenolic acid; Polymerization; Functionalization; MEDIATED FUNCTIONALIZATION; ENZYMATIC-SYNTHESIS; GALLIC ACID; ANTIOXIDANT; OXIDATION; CATECHOL;
D O I
10.1016/j.reactfunctpolym.2013.01.005
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Phenolic acids are known to possess antioxidant activities whilst chitosan is a biocompatible polymer with antibacterial activity against a broad spectrum of bacteria. Merging both types of molecules could therefore provide several potential applications. In this work, antioxidant properties of phenolic acid-functionalized-chitosan were investigated after being prepared from structurally-different phenolic acids (caffeic and gallic acids) and chitosan using the laccase from Trametes versicolor as the reaction initiator. A laccase-mediated oxidation kinetic of phenolic acids was monitored by UV-vis spectroscopy and cyclic voltammetry, as well as spin-trapping electron paramagnetic resonance spectroscopy (ST-EPR). The pH was shown to have a significant effect on the degree of phenolic acid self-polymerization, indicating the involvement of phenolate anions within the formations of coupled polyphenol products, and their functionalities, i.e. antioxidant activity. All the phenolic acid-functionalized-chitosans displayed greatly improved ABTS radical cation scavenging capacities, compared with the untreated chitosan. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1377 / 1383
页数:7
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