Non-canonical amino acids as a useful synthetic biological tool for lipase-catalysed reactions in hostile environments

被引：40

作者：

Acevedo-Rocha, Carlos G. ^{[1
,2
]}

Hoesl, Michael G. ^{[3
]}

Nehring, Sebastian ^{[3
]}

Royter, Marina ^{[4
]}

Wolschner, Christina ^{[3
]}

Wiltschi, Birgit ^{[5
]}

Antranikian, Garabed ^{[4
]}

Budisa, Nediljko ^{[3
]}

机构：

[1] Max Planck Inst Kohlenforsch, D-45470 Mulheim, Germany

[2] Univ Marburg, Fachbereich Chem, D-35032 Marburg, Germany

[3] Berlin Inst Technol, Dept Chem, Biocatalysis Grp, D-10587 Berlin, Germany

[4] Hamburg Univ Technol, Inst Tech Microbiol, D-21073 Hamburg, Germany

[5] ACIB, A-8010 Graz, Austria

来源：

CATALYSIS SCIENCE & TECHNOLOGY | 2013年 / 3卷 / 05期

关键词：

DIRECTED EVOLUTION;

D O I：

10.1039/c3cy20712a

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The incorporation of several non-canonical amino acids into the Thermoanaerobacter thermohydrosulfuricus lipase confers not only activity enhancement upon treatment with organic solvents (by up to 450%) and surfactants (resp. 1630%), but also protective effects against protein reducing (resp. 140%), alkylating (resp. 160%), and denaturing (resp. 190%) agents as well as inhibitors (resp. 40%). This approach offers novel chemically diversified biocatalysts for hostile environments.

引用

页码：1198 / 1201

页数：4

共 18 条

[1] Engineering the third wave of biocatalysis [J].

Bornscheuer, U. T. ;

Huisman, G. W. ;

Kazlauskas, R. J. ;

Lutz, S. ;

Moore, J. C. ;

Robins, K. .

NATURE, 2012, 485 (7397) :185-194

[2]

Bornscheuer UT, 2006, HYDROLASES IN ORGANIC SYNTHESIS: REGIO- AND STEREOSELECTIVE BIOTRANSFORMATIONS, 2ND EDITION, P1

[3] Prolegomena to future experimental efforts on genetic code engineering by expanding its amino acid repertoire [J].

Budisa, N .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (47) :6426-6463

[4] Directed evolution of enzyme stability [J].

Eijsink, VGH ;

Gåseidnes, S ;

Borchert, TV ;

van den Burg, B .

BIOMOLECULAR ENGINEERING, 2005, 22 (1-3) :21-30

[5] Bacterial lipases: an overview of production, purification and biochemical properties [J].

Gupta, R ;

Gupta, N ;

Rathi, P .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2004, 64 (06) :763-781

[6] Lipase Congeners Designed by Genetic Code Engineering [J].

Hoesl, Michael G. ;

Acevedo-Rocha, Carlos G. ;

Nehring, Sebastian ;

Royter, Marina ;

Wolschner, Christina ;

Wiltschi, Birgit ;

Budisa, Nediljko ;

Antranikian, Garabed .

CHEMCATCHEM, 2011, 3 (01) :213-221

[7] Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media [J].

Jin, Zi ;

Han, Shuang-Yan ;

Zhang, Li ;

Zheng, Sui-Ping ;

Wang, Yong ;

Lin, Ying .

BIORESOURCE TECHNOLOGY, 2013, 130 :102-109

[8] Improving hydrolases for organic synthesis [J].

Kazlauskas, RJ ;

Weber, HK .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1998, 2 (01) :121-126

[9] Synthesis of polymerizable sugar ester possessing long spacer catalyzed by lipase from Alcaligenes sp. and its chemical polymerization [J].

Kitagawa, M ;

Tokiwa, Y .

BIOTECHNOLOGY LETTERS, 1998, 20 (07) :627-630

[10] Improving enzymes by using them in organic solvents [J].

Klibanov, AM .

NATURE, 2001, 409 (6817) :241-246

← 1 2 →