Electrospun nanoyarn scaffold and its application in tissue engineering

被引：52

作者：

Wu, Jinglei ^{[1
]}

Liu, Shen ^{[2
]}

He, Liping ^{[1
]}

Wang, Hongsheng ^{[1
]}

He, Chuanglong ^{[1
]}

Fan, Cunyi ^{[2
]}

Me, Xiumei ^{[1
]}

机构：

[1] Donghua Univ, Coll Chem & Chem Engn & Biol Engn, Biomat & Tissue Engn Lab, Shanghai 201620, Peoples R China

[2] Shanghai Jiao Tong Univ, Dept Orthoped, Shanghai Peoples Hosp 6, Sch Med, Shanghai 200233, Peoples R China

来源：

MATERIALS LETTERS | 2012年 / 89卷

关键词：

Biomaterials; Electrospinning; Fiber technology; Nanoyarn; Tissue engineering; CELL INFILTRATION; COLLAGEN; GELATIN; MESHES;

D O I：

10.1016/j.matlet.2012.08.141

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Silk fibroin (SF)/Poly(L-lactide-co-caprolactone) P(LLA-CL) nanoyarn scaffolds were prepared by dynamic liquid electrospinning. The scaffold morphology was observed by scanning electron microscopy (SEM) and mechanical properties of the scaffold were examined. L929 mouse fibroblasts were cultured on the nanoyarn scaffolds. Cell morphology, infiltration and proliferation on the scaffolds were investigated by SEM, hematoxylin-eosin (H&E) staining and methylthiazol tetrazolium (MIT) assay, respectively. The results indicated that cells showed an organized morphology along the nanoyarns and considerable infiltration into the nanoyarn scaffolds. It was also observed that the nanoyarn scaffold significantly facilitated cell proliferation. Therefore, this work provides a promising approach to fabricate scaffolds for tissue engineering applications. (C) 2012 Published by Elsevier B.V.

引用

收藏

页码：146 / 149

页数：4

相关论文

共 13 条

[1] Progress in the Field of Electrospinning for Tissue Engineering Applications [J].

Agarwal, Seema ;

Wendorff, Joachim H. ;

Greiner, Andreas .

ADVANCED MATERIALS, 2009, 21 (32-33) :3343-3351

[2] Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffold [J].

Blakeney, Bryan A. ;

Tambralli, Ajay ;

Anderson, Joel M. ;

Andukuri, Adinarayana ;

Lim, Dong-Jin ;

Dean, Derrick R. ;

Jun, Ho-Wook .

BIOMATERIALS, 2011, 32 (06) :1583-1590

[3] Surface modification of electrospun PLLA nanofibers by plasma treatment and cationized gelatin immobilization for cartilage tissue engineering [J].

Chen, Jyh-Ping ;

Su, Chien-Hao .

ACTA BIOMATERIALIA, 2011, 7 (01) :234-243

[4] The influence of electrospun aligned poly(ε-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes [J].

Choi, Jin San ;

Lee, Sang Jin ;

Christ, George J. ;

Atala, Anthony ;

Yoo, James J. .

BIOMATERIALS, 2008, 29 (19) :2899-2906

[5] Investigating the morphological, mechanical and degradation properties of scaffolds comprising collagen, gelatin and elastin for use in soft tissue engineering [J].

Grover, Chloe N. ;

Cameron, Ruth E. ;

Best, Serena M. .

JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, 2012, 10 :62-74

[6] Electrospun materials as potential platforms for bone tissue engineering [J].

Jang, Jun-Hyeog ;

Castano, Oscar ;

Kim, Hae-Won .

ADVANCED DRUG DELIVERY REVIEWS, 2009, 61 (12) :1065-1083

[7] Geometric cues for directing the differentiation of mesenchymal stem cells [J].

Kilian, Kristopher A. ;

Bugarija, Branimir ;

Lahn, Bruce T. ;

Mrksich, Milan .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (11) :4872-4877

[8] Co-electrospun dual scaffolding system with potential for muscle-tendon junction tissue engineering [J].

Ladd, Mitchell R. ;

Lee, Sang Jin ;

Stitzel, Joel D. ;

Atala, Anthony ;

Yoo, James J. .

BIOMATERIALS, 2011, 32 (06) :1549-1559

[9] In vitro cell infiltration and in vivo cell infiltration and vascularization in a fibrous, highly porous poly(D,L-lactide) scaffold fabricated by cryogenic electrospinning technique [J].

Leong, Meng Fatt ;

Rasheed, Mohamed Zulfikar ;

Lim, Tze Chiun ;

Chian, Kerm Sin .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2009, 91A (01) :231-240

[10] Increasing the pore sizes of bone-mimetic electrospun scaffolds comprised of polycaprolactone, collagen I and hydroxyapatite to enhance cell infiltration [J].

Phipps, Matthew C. ;

Clem, William C. ;

Grunda, Jessica M. ;

Dines, Gregory A. ;

Bellis, Susan L. .

BIOMATERIALS, 2012, 33 (02) :524-534