Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion

被引:14
|
作者
Min, Seung-Ki [1 ]
Kang, Hyun Ki [2 ,3 ]
Jang, Da Hyun [2 ,3 ]
Jung, Sung Youn [2 ,3 ]
Kim, O. Bok [2 ,3 ]
Min, Byung-Moo [2 ,3 ]
Yeo, In-Sung [3 ,4 ]
机构
[1] Seoul Natl Univ, Dept Oral & Maxillofacial Surg, Sch Dent, Seoul 110749, South Korea
[2] Seoul Natl Univ, Dept Oral Biochem, Sch Dent, Seoul 110749, South Korea
[3] Seoul Natl Univ, Dent Res Inst, Seoul 110749, South Korea
[4] Seoul Natl Univ, Sch Dent, Dept Prosthodont, Seoul 110749, South Korea
基金
新加坡国家研究基金会;
关键词
ORAL IMPLANT SURFACES; HISTOMORPHOMETRIC EVALUATION; CP-TITANIUM; KNOWLEDGE;
D O I
10.1155/2013/638348
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated with a laminin-derived functional peptide, Ln2-P3 (the DLTIDDSYWYRI motif). Four Ti disc surfaces were prepared, and a human osteosarcoma (HOS) cell attachment test was performed to select two candidate surfaces for peptide coating. These two candidates were then coated with Ln2-P3 peptide, a scrambled peptide, or left uncoated to measure cell attachment to each surface, following which one surface was chosen to assess alkaline phosphatase (ALP) activity and osteogenic marker gene expression with quantitative real-time PCR. On the commercially pure Ti surface, the Ln2-P3 coating significantly increased cellular ALP activity and the expression levels of ALP and bone sialoprotein mRNA as compared with the scrambled peptide-coated and uncoated surfaces. In conclusion, although further in vivo studies are needed, the findings of this in vitro study indicate that the Ln2-P3-coated implant surface promotes bone cell adhesion, which has clinical implications for reducing the overall treatment time of dental implant therapy.
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页数:8
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