Rapid Identification of Carbapenemase Types in Enterobacteriaceae and Pseudomonas spp. by Using a Biochemical Test

被引：187
作者：
Dortet, Laurent [1 ]
Poirel, Laurent [1 ]
Nordmann, Patrice [1 ]
机构：
[1] Hop Bicetre, AP HP, Fac Med Paris Sud, Serv Bacteriol Virol,INSERM Emerging Resistance A, K Bicetre, France
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2012年 / 56卷 / 12期
关键词：
METALLO-BETA-LACTAMASES; AERUGINOSA; RESISTANCE;
D O I：
10.1128/AAC.01395-12
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A biochemical test (Carba NP test II) was developed to identify carbapenemase production in Enterobacteriaceae and Pseudomonas spp. and to discriminate between the different types of carbapenemases (classes A, B, and D). It is based on the detection of the acidification resulting from imipenem hydrolysis, coupled with tazobactam and EDTA as inhibitors. This is an easy and reliable technique (100% sensitivity and specificity) for detection of not only carbapenemase activity but also carbapenemase types in Enterobacteriaceae and Pseudomonas aeruginosa.
引用
收藏
页码：6437 / 6440
页数：4
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共 13 条
[1]   Rapid Detection of Carbapenemase-Producing Pseudomonas spp. [J].
Dortet, Laurent ;
Poirel, Laurent ;
Nordmann, Patrice .
JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (11) :3773-3776
[2]   A sensitive and specific phenotypic assay for detection of metallo-β-lactamases and KPC in Klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin [J].
Giske, C. G. ;
Gezelius, L. ;
Samuelsen, O. ;
Warner, M. ;
Sundsfjord, A. ;
Woodford, N. .
CLINICAL MICROBIOLOGY AND INFECTION, 2011, 17 (04) :552-556
[3]   Combating Antimicrobial Resistance: Policy Recommendations to Save Lives [J].
Guidos, Robert J. ;
Spellberg, Brad ;
Blaser, Martin ;
Boucher, Helen W. ;
Bradley, John S. ;
Eisenstein, Barry I. ;
Gerding, Dale ;
Lynfield, Ruth ;
Reller, L. Barth ;
Rex, John ;
Schwartz, David ;
Septimus, Edward ;
Tenover, Fred C. ;
Gilbert, David N. .
CLINICAL INFECTIOUS DISEASES, 2011, 52 :S397-S428
[4]   Metallo beta lactamases in Pseudomonas aeruginosa and Acinetobacter species [J].
Gupta, Varsha .
EXPERT OPINION ON INVESTIGATIONAL DRUGS, 2008, 17 (02) :131-143
[5]   Activities of NXL104 Combinations with Ceftazidime and Aztreonam against Carbapenemase-Producing Enterobacteriaceae [J].
Livermore, David M. ;
Mushtaq, Shazad ;
Warner, Marina ;
Zhang, Jiancheng ;
Maharjan, Sunil ;
Doumith, Michel ;
Woodford, Neil .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (01) :390-394
[6]   Pseudomonas aeruginosa:: resistance and therapeutic options at the turn of the new millennium [J].
Mesaros, N. ;
Nordmann, P. ;
Plesiat, P. ;
Roussel-Delvallez, M. ;
Van Eldere, J. ;
Glupczynski, Y. ;
Van Laethem, Y. ;
Jacobs, F. ;
Lebecque, P. ;
Malfroot, A. ;
Tulkens, P. M. ;
Van Bambeke, F. .
CLINICAL MICROBIOLOGY AND INFECTION, 2007, 13 (06) :560-578
[7]   Simple microdilution test for detection of metallo-β-lactamase production in Pseudomonas aeruginosa [J].
Migliavacca, R ;
Docquier, JD ;
Mugnaioli, C ;
Amicosante, G ;
Daturi, R ;
Lee, KW ;
Rossolini, GM ;
Pagani, L .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (11) :4388-4390
[8]   Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues [J].
Miriagou, V. ;
Cornaglia, G. ;
Edelstein, M. ;
Galani, I. ;
Giske, C. G. ;
Gniadkowski, M. ;
Malamou-Lada, E. ;
Martinez-Martinez, L. ;
Navarro, F. ;
Nordmann, P. ;
Peixe, L. ;
Pournaras, S. ;
Rossolini, G. M. ;
Tsakris, A. ;
Vatopoulos, A. ;
Canton, R. .
CLINICAL MICROBIOLOGY AND INFECTION, 2010, 16 (02) :112-122
[9]   Identification and screening of carbapenemase-producing Enterobacteriaceae [J].
Nordmann, P. ;
Gniadkowski, M. ;
Giske, C. G. ;
Poirel, L. ;
Woodford, N. ;
Miriagou, V. .
CLINICAL MICROBIOLOGY AND INFECTION, 2012, 18 (05) :432-438
[10]   Rapid Detection of Carbapenemase-producing Enterobacteriaceae [J].
Nordmann, Patrice ;
Poirel, Laurent ;
Dortet, Laurent .
EMERGING INFECTIOUS DISEASES, 2012, 18 (09) :1503-1507
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