Preparation of N2-Ethyl-2′-deoxyguanosine-d4 as an Internal Standard for the Electrospray Ionization-Tandem Mass Spectrometric Determination of DNA Damage by Acetaldehyde

被引：3

作者：

Esaka, Yukihiro ^{[1
,2
]}

Aruga, Hiromitsu ^{[1
]}

Kunishima, Saki ^{[1
]}

Yamamoto, Takuhei ^{[1
]}

Murakami, Hiroya ^{[3
]}

Sawama, Yoshinari ^{[1
]}

Sajiki, Hironao ^{[1
]}

Uno, Bunji ^{[1
,2
]}

机构：

[1] Gifu Pharmaceut Univ, 1-25-4 Daigaku Nishi, Gifu 5011196, Japan

[2] Gifu Univ, United Grad Sch Drug Discovery & Med Informat Sci, 1-1 Yanagido, Gifu 5011194, Japan

[3] Aichi Inst Technol, Dept Appl Chem, 1247 Yachigusa,Yakusa Cho, Toyota 4700392, Japan

来源：

ANALYTICAL SCIENCES | 2020年 / 36卷 / 07期

基金：

日本学术振兴会;

关键词：

N-2-Ethyl-2 '-deoxyguanosine-d(4); isotopic internal standard; ESI-MS/MS; acetaldehyde-d(4); D2O; cancer risk marker; ADDUCTS; NUCLEOSIDES;

D O I：

10.2116/analsci.19N034

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The deuteration of N-2-ethyl-2'-deoxyguanosine (Et-dG), which is a DNA adduct generated from acetaldehyde, was studied by the addition reaction of acetaldehyde-d(4 )to 2'-deoxyguanosine (dG) in deuterium oxide (D2O), with the aim to obtain an isotope internal standard for the liquid chromatography/tandem mass spectrometry (LC/MS/MS) quantitation of Et-dG. The replacement of the dG C-8 hydrogen atom by a deuteron atom took place at 50 degrees C in D2O and afforded a mixture of Et-dG-d(4) and Et-dG-d(5). Et-dG-d(4), which was stable in aqueous solutions, was prepared by incubating the mixture in H2O at 60 degrees C for 48 h. The calibration curve was obtained by multiple reaction monitoring (MRM) measurements using a hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometric (HILIC/ESI-MS/MS) system between the Et-dG concentration, ranging from 1.0 x 10(-10) to 4.0 x 10(-9 )M in the sample solutions, and the relative peak areas of Et-dG (m/z: 296.1 -> 180.1) to the value of Et-dG-d(4) (m/z: 300.2 -> 184.2), with an internal standard showing good linearity (R-2 = 0.995, n = 5).

引用

页码：877 / 880

页数：4