Holographic photolysis of caged neurotransmitters

被引：173

作者：

Lutz, Christoph ^{[1
]}

Otis, Thomas S. ^{[2
,3
]}

DeSars, Vincent ^{[1
]}

Charpak, Serge ^{[1
]}

DiGregorio, David A. ^{[2
]}

Emiliani, Valentina ^{[1
]}

机构：

[1] Univ Paris 05, INSERM, Neurophysiol & New Microscopy Lab, CNRS,UMR 8154,U603, F-75270 Paris 06, France

[2] Univ Paris 05, CNRS, Lab Brain Physiol, UMR 8118, F-75270 Paris 06, France

[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Neurobiol, Los Angeles, CA 90095 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 09期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/NMETH.1241

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Stimulation of light-sensitive chemical probes has become a powerful tool for the study of dynamic signaling processes in living tissue. Classically, this approach has been constrained by limitations of lens-based and point-scanning illumination systems. Here we describe a microscope configuration that incorporates a nematic liquid-crystal spatial light modulator to generate holographic patterns of illumination. This microscope can produce illumination spots of variable size and number, and in patterns shaped to precisely match user-defined elements in a specimen. Using holographic illumination to photolyze caged glutamate in brain slices, we show that shaped excitation on segments of neuronal dendrites and simultaneous, multispot excitation of different dendrites enables precise spatial and rapid temporal control of glutamate receptor activation. By allowing the excitation volume shape to be tailored precisely, the holographic microscope provides an extremely flexible method for activation of various photosensitive proteins and small molecules.

引用

页码：821 / 827

页数：7

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