Characterization and promoter activity of chromoplast specific carotenoid associated gene (CHRC) from Oncidium Gower!Ramsey

被引：23

作者：

Chiou, Chung-Yi ^{[1
]}

Wu, Keqiang ^{[1
]}

Yeh, Kai-Wun ^{[1
]}

机构：

[1] Natl Taiwan Univ, Coll Life Sci, Inst Plant Biol, Taipei 106, Taiwan

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 10期

关键词：

carotenoids; flower colour; orchid (Oncidium); plant pigments; tissue-specific promoter;

D O I：

10.1007/s10529-008-9767-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tissue-specific promoters are required for plant molecular breeding to drive a target gene in the appropriate location in plants. A chromoplast-specific, carotenoid-associated gene (OgCHRC) and its promoter (Pchrc) were isolated from Oncidium orchid and characterized. Northern blot analysis revealed that OgCHRC is specifically expressed in flowers, not in roots and leaves. Transient expression assay of Pchrc by bombardment transformation confirmed its differential expression pattern in floral tissues of different horticulture plants and cell-type location in conical papillate cells of adaxial epidermis of flower. These results suggest that Pchrc could serve as a useful tool in ornamental plant biotechnology to modify flower color.

引用

页码：1861 / 1866

页数：6