In Vitro and In Vivo Survival and Colonic Adhesion of Pediococcus acidilactici MTCC5101 in Human Gut

被引：18
作者：
Balgir, Praveen P. [1 ]
Kaur, Baljinder [1 ]
Kaur, Tejinder [1 ]
Daroch, Natisha [1 ]
Kaur, Gurpreet [1 ]
机构：
[1] Punjabi Univ, Dept Biotechnol, Patiala 147002, Punjab, India
来源：
BIOMED RESEARCH INTERNATIONAL | 2013年 / 2013卷
关键词：
LACTIC-ACID BACTERIA; LACTOBACILLUS-CASEI; STRAIN GG; STREPTOCOCCUS-THERMOPHILUS; GASTROINTESTINAL-TRACT; ESCHERICHIA-COLI; PROBIOTIC STRAIN; DOSE-RESPONSE; BIFIDOBACTERIUM; SAFETY;
D O I：
10.1155/2013/583850
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The present study aims to investigate the probiotic nature of Pediococcus acidilactici MTCC5101 by an in vitro assay of bacterial adherence to intestinal epithelial cells of human gastrointestinal (GI) tract using Caco-2 cell line. Further to assess the in vivo survival in the GI tract, oral feeding was carried out with the help of 10 healthy volunteers. The effect on wellness was assessed by studying blood biochemical parameters of the volunteers. The survival of the bacteria was assessed using PCR-based detection of P. acidilactici MTCC5101 in fecal samples. The probiotic nature of P. acidilactici MTCC 5101 was strengthened by its adherence to the intestinal epithelial Caco-2 cell line in the in vitro SEM observations. Oral feeding study for assessing the survival of bacteria in GI tract of volunteers showed the strain to be established in the GI tract which survived for about 2 weeks after feeding.
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页数：9
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