Development and characterization of microsatellite markers in Zanthoxylum ailanthoides (Rutaceae)

被引:5
作者
Nagai, H. [1 ]
Yoshida, T. [1 ]
Kamiya, K. [1 ]
Yahara, T. [1 ]
Tachida, H. [1 ]
机构
[1] Kyushu Univ, Fac Sci, Dept Biol, Chuo Ku, Fukuoka 8108560, Japan
基金
日本学术振兴会;
关键词
microsatellite; prickly ash; population structure; Zanthoxylum ailanthoides;
D O I
10.1111/j.1755-0998.2008.02507.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nine microsatellite markers were developed for Zanthoxylum ailanthoides, a typical pioneer tree. Averaged over the nine loci, the number of alleles per locus was 5.1. The observed and expected heterozygosities ranged from 0.233 to 0.833 and from 0.314 to 0.823, with averages of 0.606 and 0.641, respectively. No loci showed significant departures from Hardy-Weinberg equilibrium or linkage equilibrium after Bonferroni correction (P > 0.05). These markers will be useful for parentage analyses and studies of population genetic structure in the species.
引用
收藏
页码:667 / 669
页数:3
相关论文
共 8 条
[1]  
AMADOR DM, 1998, WORKSH MAN U FLOR GA
[2]   Rapid isolation of CA microsatellites from the tilapia genome [J].
Carleton, KL ;
Streelman, JT ;
Lee, BY ;
Garnhart, N ;
Kidd, M ;
Kocher, TD .
ANIMAL GENETICS, 2002, 33 (02) :140-144
[3]   High population differentiation and unusual haplotype structure in a shade-intolerant pioneer tree species, Zanthoxylum ailanthoides (Rutaceae) revealed by analysis of DNA polymorphism at four nuclear loci [J].
Kamiya, K. ;
Moritsuka, E. ;
Yoshida, T. ;
Yahara, T. ;
Tachida, H. .
MOLECULAR ECOLOGY, 2008, 17 (10) :2329-2338
[4]   GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research [J].
Peakall, R ;
Smouse, PE .
MOLECULAR ECOLOGY NOTES, 2006, 6 (01) :288-295
[5]   GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].
RAYMOND, M ;
ROUSSET, F .
JOURNAL OF HEREDITY, 1995, 86 (03) :248-249
[6]  
Rozen S, 2000, PRIMER 3
[7]   CHLOROPLAST DNA POLYMORPHISMS IN LODGEPOLE AND JACK PINES AND THEIR HYBRIDS [J].
WAGNER, DB ;
FURNIER, GR ;
SAGHAIMAROOF, MA ;
WILLIAMS, SM ;
DANCIK, BP ;
ALLARD, RW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (07) :2097-2100
[8]  
WEIR BS, 1984, EVOLUTION, V38, P1358, DOI [10.2307/2408641, 10.1111/j.1558-5646.1984.tb05657.x]