Co-expression of Piwil2/Piwil4 in nucleus indicates poor prognosis of hepatocellular carcinoma

被引:17
作者
Zeng, Guangping [1 ]
Zhang, Deying [1 ]
Liu, Xing [1 ]
Kang, Qing [1 ]
Fu, Yiyao [1 ]
Tang, Bo [1 ]
Guo, Wenhao [1 ]
Zhang, Yuanyuan [2 ]
Wei, Guanghui [1 ]
He, Dawei [1 ]
机构
[1] Chongqing Med Univ, Childrens Hosp,Dept Urol, Chongqing Int Sci & Technol,Cooperat Ctr Child De, Minist Educ,Key Lab Child Dev & Disorders,Key Lab, Chongqing 400014, Peoples R China
[2] Wake Forest Univ, Sch Med, Wake Forest Inst Regenerat Med, Med Ctr Blvd, Winston Salem, NC 27103 USA
基金
中国国家自然科学基金;
关键词
hepatocellular carcinoma; molecular chaperone; Piwil2; Piwil4; prognosis; PIWI PROTEINS; STEM-CELLS; ARGONAUTE FAMILY; COLON-CANCER; GENE; RNAI; TUMORIGENESIS; METASTASIS; MEMBERS; MILI;
D O I
10.18632/oncotarget.13491
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Purpose: This study aimed to explore the localization and expression of P-element-induced wimpy testis-like 2 (piwil2)/Piwil4 in hepatocellular carcinoma (HCC) tissues, and analyze the correlation between co-expression pattern and prognosis of HCC. Results: Piwil2 showed 100% positive expression in the cell nucleus, with the intensity higher than in the cytoplasm. Piwil4 showed a lower intensity of expression in the cell nucleus than in the cytoplasm. The molecular chaperone Piwil2/Piwil4 had four co-expression patterns: nuclear co-expression, nuclear and cytoplasmic co-expression, cytoplasmic co-expression, and non-coexpression. The survival rate and the overall survival sequentially increased. The prognostic phenotype of the nuclear co-expression of Piwil2/Piwil4 was worse than that of non-coexpression, and the intracellular localization and expression of Piwil2 and Piwil4 were not significantly different. Methods: HCC pathological tissue samples with follow-up information (90 cases) and 2 normal control liver tissues were collected and made into a 92-site microarray. The expression of Piwil2 and Piwil4 was detected using the immunofluorescence double staining method. The differences in the expression and location of Piwil2 and Piwil4 in tumor cells were explored, and the influence of such differences on the long-term survival rate of HCC was studied using Kaplan-Meier survival curve and log-rank test. The clinical staging was analyzed according to the HCC international TNM staging criteria. Conclusions: The nuclear co-expression of Piwil2/Piwil4 indicated that patients with HCC had a worse prognostic phenotype. The molecular chaperone Piwil2/Piwil4 seems promising as a molecular marker for prognosis judgment; a single marker (Piwil2/Piwil4) cannot be used for prognosis judgment.
引用
收藏
页码:4607 / 4617
页数:11
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