Hair follicle stem cell cultures reveal self-organizing plasticity of stem cells and theirprogeny

被引:65
|
作者
Chacon-Martinez, Carlos Andres [1 ]
Klose, Markus [2 ]
Niemann, Catherin [3 ,4 ]
Glauche, Ingmar [2 ]
Wickstroem, Sara A. [1 ,5 ]
机构
[1] Max Planck Inst Biol Ageing, Paul Gerson Unna Grp Skin Homeostasis & Ageing, Cologne, Germany
[2] Tech Univ Dresden, Inst Med Informat & Biometry, Carl Gustav Carus Fac Med, Dresden, Germany
[3] Univ Cologne, Inst Biochem 2, Fac Med, Cologne, Germany
[4] Univ Cologne, Ctr Mol Med Cologne, Cologne, Germany
[5] Univ Cologne, Cologne Excellence Cluster Cellular Stress Respon, Cologne, Germany
来源
EMBO JOURNAL | 2017年 / 36卷 / 02期
关键词
differentiation; hair follicle stem cells; niche; reprogramming; stem cell cultures; NICHE; DIFFERENTIATION; EXPRESSION; ENRICHMENT; MECHANISM; DYNAMICS; GROWTH;
D O I
10.15252/embj.201694902
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Understanding how complex tissues are formed, maintained, and regenerated through local growth, differentiation, and remodeling requires knowledge on how single-cell behaviors are coordinated on the population level. The self-renewing hair follicle, maintained by a distinct stem cell population, represents an excellent paradigm to address this question. A major obstacle in mechanistic understanding of hair follicle stem cell (HFSC) regulation has been the lack of a culture system that recapitulates HFSC behavior while allowing their precise monitoring and manipulation. Here, we establish an invitro culture system based on a 3D extracellular matrix environment and defined soluble factors, which for the first time allows expansion and long-term maintenance of murine multipotent HFSCs in the absence of heterologous cell types. Strikingly, this scheme promotes de novo generation of HFSCs from non-HFSCs and vice versa in a dynamic self-organizing process. This bidirectional interconversion of HFSCs and their progeny drives the system into a population equilibrium state. Our study uncovers regulatory dynamics by which phenotypic plasticity of cells drives population-level homeostasis within a niche, and provides a discovery tool for studies on adult stem cell fate.
引用
收藏
页码:151 / 164
页数:14
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