A miniature fiber optic surface plasmon resonance sensor for fast detection of staphylococcal enterotoxin B

被引：149
|
作者：
Slavík, R
Homola, J
Brynda, E
机构：
[1] Acad Sci Czech Republ, Inst Radio Engn & Elect, CR-18251 Prague, Czech Republic
[2] Acad Sci Czech Republ, Inst Macromol Chem, CR-16206 Prague, Czech Republic
来源：
BIOSENSORS & BIOELECTRONICS | 2002年 / 17卷 / 6-7期
关键词：
biosensor; optical fiber sensor; surface plasmon resonance; foodborne pathogens; staphylococcal enterotoxin B;
D O I：
10.1016/S0956-5663(02)00013-1
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A fiber optic surface plasmon resonance (SPR) biosensor for detection of Staphylococcal enterotoxin B (SEB) is reported. The sensor is based on spectral interrogation of surface plasmons in a miniature sensing element based on a side-polished single-mode optical fiber with a thin metal overlayer. For specific detection of SEB, the SPR sensor is functionalized with a covalently crosslinked double-layer of antibodies against SEB. The SPR biosensor is demonstrated to be able to detect ng/ml concentrations of SEB in less than 10 min. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：591 / 595
页数：5
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