A miniature fiber optic surface plasmon resonance sensor for fast detection of staphylococcal enterotoxin B

被引：150
作者：
Slavík, R
Homola, J
Brynda, E
机构：
[1] Acad Sci Czech Republ, Inst Radio Engn & Elect, CR-18251 Prague, Czech Republic
[2] Acad Sci Czech Republ, Inst Macromol Chem, CR-16206 Prague, Czech Republic
来源：
BIOSENSORS & BIOELECTRONICS | 2002年 / 17卷 / 6-7期
关键词：
biosensor; optical fiber sensor; surface plasmon resonance; foodborne pathogens; staphylococcal enterotoxin B;
D O I：
10.1016/S0956-5663(02)00013-1
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A fiber optic surface plasmon resonance (SPR) biosensor for detection of Staphylococcal enterotoxin B (SEB) is reported. The sensor is based on spectral interrogation of surface plasmons in a miniature sensing element based on a side-polished single-mode optical fiber with a thin metal overlayer. For specific detection of SEB, the SPR sensor is functionalized with a covalently crosslinked double-layer of antibodies against SEB. The SPR biosensor is demonstrated to be able to detect ng/ml concentrations of SEB in less than 10 min. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：591 / 595
页数：5
相关论文
共 20 条
[1]  
ARCHER D L, 1988, Clinical Microbiology Reviews, V1, P377
[2]  
BERGDOLL MS, 1991, J ASSOC OFF ANA CHEM, V74, P706
[3]  
Boisde G, 1996, CHEM BIOCH SENSING O
[4]   Immobilisation of multilayer bioreceptor assemblies on solid substrates [J].
Brynda, E ;
Houska, M ;
Skvor, J ;
Ramsden, JJ .
BIOSENSORS & BIOELECTRONICS, 1998, 13 (02) :165-172
[5]   Tuning of spectral operation range of a waveguide surface plasmon resonance sensor [J].
Ctyroky, J ;
Homola, J ;
Skalsky, M .
ELECTRONICS LETTERS, 1997, 33 (14) :1246-1248
[6]   ESTIMATION OF HUMAN DOSE OF STAPHYLOCOCCAL ENTEROTOXIN-A FROM A LARGE OUTBREAK OF STAPHYLOCOCCAL FOOD POISONING INVOLVING CHOCOLATE MILK [J].
EVENSON, ML ;
HINDS, MW ;
BERNSTEIN, RS ;
BERGDOLL, MS .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1988, 7 (04) :311-316
[7]   WAVE-GUIDE SURFACE-PLASMON RESONANCE SENSORS [J].
HARRIS, RD ;
WILKINSON, JS .
SENSORS AND ACTUATORS B-CHEMICAL, 1995, 29 (1-3) :261-267
[8]   Surface plasmon resonance sensors: review [J].
Homola, J ;
Yee, SS ;
Gauglitz, G .
SENSORS AND ACTUATORS B-CHEMICAL, 1999, 54 (1-2) :3-15
[9]   Fibre-optic sensor based on surface plasmon resonance [J].
Homola, J ;
Slavik, R .
ELECTRONICS LETTERS, 1996, 32 (05) :480-482
[10]   A FIBEROPTIC CHEMICAL SENSOR-BASED ON SURFACE-PLASMON RESONANCE [J].
JORGENSON, RC ;
YEE, SS .
SENSORS AND ACTUATORS B-CHEMICAL, 1993, 12 (03) :213-220
12