Densitometric-High-Performance Thin-Layer Chromatographic Estimation of Diosmin, Hesperidin, and Ascorbic Acid in Pharmaceutical Formulations

被引：8
|
作者：
Al-Taweel, Areej M. [1 ]
Alqasoumi, Saleh I. [1 ]
Alam, Prawez [2 ]
Abdel-Kader, Maged S. [2 ,3 ]
机构：
[1] King Saud Univ, Dept Pharmacognosy, Coll Pharm, Riyadh 11451, Saudi Arabia
[2] Salman Bin Abdulaziz Univ, Dept Pharmacognosy, Coll Pharm, Al Kharj 11942, Saudi Arabia
[3] Univ Alexandria, Dept Pharmacognosy, Coll Pharm, Alexandria 21215, Egypt
来源：
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC | 2013年 / 26卷 / 04期
关键词：
Diosmin; Hesperidin; Ascorbic acid; HPTLC-densitometry; ICH guidelines; Qualitative; Quantitative; CHRONIC VENOUS INSUFFICIENCY; DAFLON; 500; MG; LIQUID-CHROMATOGRAPHY; QUANTITATIVE-ANALYSIS; UV DETECTION; NARINGIN; FRUIT; FLAVONOIDS; HESPERETIN; EXTRACTS;
D O I：
10.1556/JPC.26.2013.4.8
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An attempt was made to develop and validate a simple, accurate high-performance thin-layer chromatographic (HPTLC) method for the simultaneous determination of diosmin, hesperidin, and ascorbic acid in pharmaceutical preparations. Analysis was performed on 10 cm x 20 cm aluminum-backed plates coated with 0.2 mm layers of silica gel 60 F-254 (Merck, Germany). CAMAG TLC Scanner III was used for UV densitometric scanning. The used HPTLC system was found to give sharp, symmetrical, and well-resolved peaks at R-F values of 0.34 +/- 0.02, 0.40 +/- 0.04, and 0.56 +/- 0.03, and linearity was found in the ranges 100-800 ng/spot (r(2) = 0.9985), 100-800 ng/spot (r(2) = 0.9966), and 50-400 ng/spot (r(2) = 0.9951) for diosmin, hesperidin, and ascorbic acid, respectively.
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页码：336 / 342
页数：7
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