Enhancing the performance of fluorescence emission difference microscopy using beam modulation

被引:27
作者
Li, Shuai [1 ]
Kuang, Cuifang [1 ]
Hao, Xiang [1 ]
Wang, Yifan [1 ]
Ge, Jianhong [1 ]
Liu, Xu [1 ]
机构
[1] Zhejiang Univ, State Key Lab Modern Opt Instrumentat, Hangzhou 310027, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
diffraction theory; microscopy; resolution; CONFOCAL MICROSCOPY; RESOLUTION LIMIT; SUPERRESOLUTION; DIFFRACTION; SPOT;
D O I
10.1088/2040-8978/15/12/125708
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Fluorescence emission difference microscopy (FED) has been proved to be an effective method to break the diffraction barrier which had restricted the spatial resolution of optical microscopy in the far-field for a long time. In this paper, we theoretically demonstrate that the attainable resolution of FED can be further enhanced by using beam modulation. By applying beam shape modulating, polarization state modulating, phase encoding and beam blocking to the illumination beams in the process of FED imaging, we optimize the size of the corresponding PSFs and thus improve the resolving ability of FED. The simulation tests demonstrate that in the optimal case after beam modulation, the resolution of FED can be enhanced by a factor of 26% compared with a conventional one.
引用
收藏
页数:8
相关论文
共 32 条
[1]  
Abbe E., 1873, Archiv f ur Mikroskopische Anatomie, V9, P413, DOI DOI 10.1007/BF02956173
[2]   Harmonic generation in gases by use of Bessel-Gauss laser beams [J].
Altucci, C ;
Bruzzese, R ;
D'Antuoni, D ;
de Lisio, C ;
Solimeno, S .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS, 2000, 17 (01) :34-42
[3]  
[Anonymous], 2000, Principles of optics: electromagnetic theory of propagation, interference and diffraction of light
[4]   Conformational analysis of single polymer chains in three dimensions by super-resolution fluorescence microscopy [J].
Aoki, Hiroyuki ;
Mori, Kazuki ;
Ito, Shinzaburo .
SOFT MATTER, 2012, 8 (16) :4390-4395
[5]   Imaging intracellular fluorescent proteins at nanometer resolution [J].
Betzig, Eric ;
Patterson, George H. ;
Sougrat, Rachid ;
Lindwasser, O. Wolf ;
Olenych, Scott ;
Bonifacino, Juan S. ;
Davidson, Michael W. ;
Lippincott-Schwartz, Jennifer ;
Hess, Harald F. .
SCIENCE, 2006, 313 (5793) :1642-1645
[6]   Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules [J].
Burnette, Dylan T. ;
Sengupta, Prabuddha ;
Dai, Yuhai ;
Lippincott-Schwartz, Jennifer ;
Kachar, Bechara .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (52) :21081-21086
[7]   Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI) [J].
Dertinger, T. ;
Colyer, R. ;
Iyer, G. ;
Weiss, S. ;
Enderlein, J. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (52) :22287-22292
[8]  
GALLINA M. E., 2013, OPT NANOSC, V2, P1
[9]   BESSEL-GAUSS BEAMS [J].
GORI, F ;
GUATTARI, G ;
PADOVANI, C .
OPTICS COMMUNICATIONS, 1987, 64 (06) :491-495
[10]   Scanning confocal optical microscopy and magnetic resonance on single defect centers [J].
Gruber, A ;
Drabenstedt, A ;
Tietz, C ;
Fleury, L ;
Wrachtrup, J ;
vonBorczyskowski, C .
SCIENCE, 1997, 276 (5321) :2012-2014