Characterization of polysaccharides with antioxidant and immunological activities from Rhizoma Acori Tatarinowii

被引：62
作者：
Zhang, Wuxia [1 ]
Song, Dan [1 ]
Xu, Dan [1 ]
Wang, Tingting [1 ]
Chen, Lu [1 ]
Duan, Jinyou [1 ]
机构：
[1] Northwest A&F Univ, Coll Sci, Yangling 712100, Shaanxi, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2015年 / 133卷
基金：
中国国家自然科学基金;
关键词：
Rhizoma Acori Tatarinowii; Polysaccharides; Antioxidative activity; Immunological activity; STRUCTURAL-CHARACTERIZATION; NITRIC-OXIDE; APOPTOSIS; CELLS; PROTECTS; INSIGHTS; ACID;
D O I：
10.1016/j.carbpol.2015.07.018
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Rhizoma Acori Tatarinowii is widely used in traditional Chinese medicine. In this study, three novel polysaccharides designated RATPW, RATPS1 and RATPS2 were isolated from Rhizoma Acori Tatarinowii by DEAE-52 cellulose chromatography. Their structures were characterized using physicochemical and spectral methods. Chemical analysis indicated that RATPW (6.5 x 10(3) Da) mainly composed of glucose and fructose. RATPS1 (1.5 x 10(5) Da) contained galactose and arabinose, while RATPS2 (5.3 x 10(4) Da) contained similar to 49.5% galacturonic acid along with rhamnose, fructose, galactose, and arabinose. In vitro, RATPS2 showed the most significant scavenging activity on DPPH and hydroxyl radical. Three polysaccharides could protect the PC12 cells from H2O2-induced damage. Immunological tests indicated that both RATPW and RATPS2 significantly stimulated NO production and phagocytic activity in RAW264.7, and promoted splenocyte proliferation. These data suggested that polysaccharides RATPW and RATPS2 had the potential as novel natural sources of antioxidative and immunopotentiating agents. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：154 / 162
页数：9
相关论文
共 55 条
[1]   Ghrelin and des-acyl ghrelin inhibit cell death in cardiomyocytes and endothelial cells through ERK1/2 and PI 3-kinase/AKT [J].
Baldanzi, G ;
Filigheddu, N ;
Cutrupi, S ;
Catapano, F ;
Bonissoni, S ;
Fubini, A ;
Malan, D ;
Baj, G ;
Granata, R ;
Broglio, F ;
Papotti, M ;
Surico, N ;
Bussolino, F ;
Isgaard, J ;
Deghenghi, R ;
Sinigaglia, F ;
Prat, M ;
Muccioli, G ;
Ghigo, E ;
Graziani, A .
JOURNAL OF CELL BIOLOGY, 2002, 159 (06) :1029-1037
[2]   NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS [J].
BLUMENKR.N ;
ASBOEHAN.G .
ANALYTICAL BIOCHEMISTRY, 1973, 54 (02) :484-489
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   Oxidative stress in Alzheimer's disease brain: New insights from redox proteomics [J].
Butterfield, D. Allan ;
Perluigi, Marzia ;
Sultana, Rukhsana .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2006, 545 (01) :39-50
[5]   Effect of abietane diterpenes from Plectranthus grandidentatus on T- and B-lymphocyte proliferation [J].
Cerqueira, F ;
Cordeiro-Da-Silva, A ;
Gaspar-Marques, C ;
Simoes, F ;
Pinto, MMM ;
Nascimento, MSJ .
BIOORGANIC & MEDICINAL CHEMISTRY, 2004, 12 (01) :217-223
[6]   A polysaccharide from Sargassum fusiforme protects against immunosuppression in cyclophosphamide-treated mice [J].
Chen, Xiaoming ;
Nie, Wenjian ;
Fan, Sairong ;
Zhang, Junfeng ;
Wang, Yanxing ;
Lu, Jianxin ;
Jin, Liqin .
CARBOHYDRATE POLYMERS, 2012, 90 (02) :1114-1119
[7]   Immune cell stimulating activity of mucopolysaccharide isolated from yam (Dioscorea batatas) [J].
Choi, EM ;
Koo, SJ ;
Hwang, JK .
JOURNAL OF ETHNOPHARMACOLOGY, 2004, 91 (01) :1-6
[8]   Chemical characterization of the immunomodulating polysaccharide of Aloe vera L. [J].
Chow, JTN ;
Williamson, DA ;
Yates, KM ;
Goux, WJ .
CARBOHYDRATE RESEARCH, 2005, 340 (06) :1131-1142
[9]   Nitric oxide in immunity and inflammation [J].
Coleman, JW .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2001, 1 (08) :1397-1406
[10]   G1-4A, an immunomodulatory polysaccharide from Tinospora cordifolia, modulates macrophage responses and protects mice against lipopolysaccharide induced endotoxic shock [J].
Desai, Veena R. ;
Ramkrishnan, Rupal ;
Chintalwar, Gajanan J. ;
Sainis, K. B. .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2007, 7 (10) :1375-1386
123456