Nicotinamide Mononucleotide Supplementation Reverses the Declining Quality of Maternally Aged Oocytes

被引:234
作者
Miao, Yilong [1 ]
Cui, Zhaokang [1 ]
Gao, Qian [2 ]
Rui, Rong [2 ]
Xiong, Bo [1 ]
机构
[1] Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing 210095, Peoples R China
[2] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China
基金
中国博士后科学基金; 中国国家自然科学基金;
关键词
HIGH-FAT DIET; OXIDATIVE STRESS; NAD(+); METABOLISM; FERTILITY; APOPTOSIS; OLD;
D O I
10.1016/j.celrep.2020.107987
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Advanced maternal age is highly associated with a decline in oocyte quality, but effective approaches to improve it have still not been fully determined. Here, we report that in vivo supplementation of nicotinamide mononucleotide (NMN) efficaciously improves the quality of oocytes from naturally aged mice by recovering nicotinamide adenine dinucleotide (NAD(+)) levels. NMN supplementation not only increases ovulation of aged oocytes but also enhances their meiotic competency and fertilization ability by maintaining the normal spindle/chromosome structure and the dynamics of the cortical granule component ovastacin. Moreover, single-cell transcriptome analysis shows that the beneficial effect of NMN on aged oocytes is mediated by restoration of mitochondrial function, eliminating the accumulated ROS to suppress apoptosis. Collectively, our data reveal that NMN supplementation is a feasible approach to protect oocytes from advanced maternal age-related deterioration, contributing to the improvement of reproductive outcome of aged women and assisted reproductive technology.
引用
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页数:16
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