Evaluation of the use of plasma preparation tubes for HIV viral load testing on the COBAS AmpliPrep/COBAS TaqMan HIV-1 version 2.0

被引：3
|
作者：
Goedhals, Dominique [1 ]
Scott, Lesley E. [2 ]
Moretti, Serena [1 ,3 ]
Cooper, Mark A. [1 ]
Opperman, Willem J. L. [1 ]
Rossouw, Inez [1 ]
机构：
[1] Univ Orange Free State, Natl Hlth Lab Serv, Dept Med Microbiol & Virol, ZA-9300 Bloemfontein, South Africa
[2] Univ Witwatersrand, Sch Pathol, Fac Hlth Sci, Dept Mol Med & Haematol, Johannesburg, South Africa
[3] Univ Orange Free State, Dept Microbial Biochem & Food Biotechnol, ZA-9300 Bloemfontein, South Africa
来源：
JOURNAL OF VIROLOGICAL METHODS | 2013年 / 187卷 / 02期
关键词：
HIV viral load; PPT; CAP/CTM HIV-2 v2; IMMUNODEFICIENCY-VIRUS RNA; ANTIRETROVIRAL THERAPY; TYPE-1; RNA; ASSAYS; QUANTIFICATION; COLLECTION; STANDARD; SAMPLES;
D O I：
10.1016/j.jviromet.2012.11.019
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
HIV viral load monitoring forms an essential part of the management of patients receiving antiretroviral therapy, but transport of samples without loss of RNA integrity may be problematic in resource limited settings. The use of plasma preparation tubes (PPT) which can be centrifuged to separate cellular components before transport may provide a simple and cost-effective alternative to standard EDTA samples. We investigated whether PPT generated reliable results using the COBAS (R) AmpliPrep/COBAS (R) TaqMan (R) HIV-1 test version 2.0 (CAP/CTM HIV-1 v2.0). The mean difference between EDTA and PPT prepared samples (n = 261) was acceptable (log 0.04 copies/ml, percentage similarity CV 3.53%). PPT can be used for viral load testing on the CAP/CTM HIV-1 v2.0. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：248 / 250
页数：3
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