Macromolecular crystal annealing: evaluation of techniques and variables

被引：54

作者：

Harp, JM ^{[1
]}

Hanson, BL

Timm, DE

Bunick, GJ

机构：

[1] Univ Tennessee, Grad Sch Biomed Sci, Oak Ridge, TN 37831 USA

[2] Indiana Univ, Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN USA

[3] Oak Ridge Natl Lab, Div Life Sci, Oak Ridge, TN 37831 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷

关键词：

D O I：

10.1107/S0907444999005442

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Additional examples of successful application of macromolecular crystal annealing are presented. A qualitative evaluation of variables related to the annealing process was conducted using a variety of macromolecular crystals to determine in which cases parameters may be varied and in which cases the original macromolecular crystal annealing protocol is preferred. A hypothesis is presented relating the solvent content of the crystal to the specific protocol necessary for the successful application of annealing.

引用

页码：1329 / 1334

页数：6

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