Fabrication of β-chitosan nanoparticles and its anticancer potential against human hepatoma cells

beta-Chitosan from the gladius was enzymatically depolymerized and utilized for the synthesis of beta-chitosan nanoparticles using sodium tripolyphosphate by ionotropic gelation. The size and zeta potential of beta-Chitosan nanoparticles (beta CNP) were determined. The structural features were evaluated by FT-IR and NMR spectral analysis. The morphological characterization, composition and surface topography of beta-CNP were explored by SEM, EDAX and AFM techniques. The thermal and crystallographic nature of beta-CNP was also studied. The cell viability of HepG2 cells inhibited by beta-CNP was detected in a dose-dependent manner. The inhibitory concentration of beta-CNP was 30 mu g/ml. Various biochemical parameters such as TBARS and lipid hydroperoxides, enzymatic and non-enzymatic antioxidant (SOD, CAT, GPx and GSH) studies proved the anticancer property of beta-CNP in HepG2 cells. This study suggests that beta-CNP should be a promising drug for treating hepatocellular carcinoma in future. (C) 2016 Published by Elsevier B.V.