Acidulated Phosphate Fluoride Application Changes the Protein Composition of Human Acquired Enamel Pellicle

被引：9
作者：
Masson, N. [1 ,2 ]
Domingues, R. R. [2 ]
Cury, J. A. [1 ]
Paes Leme, A. F. [2 ]
机构：
[1] Univ Estadual Campinas, Piracicaba Dent Sch, UNICAMP, Piracicaba, Brazil
[2] CNPEM, Brazilian Biosci Natl Lab, LNBio, Mass Spectrometry Lab, BR-13083970 Campinas, SP, Brazil
来源：
CARIES RESEARCH | 2013年 / 47卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
Acidulated phosphate fluoride; Dental pellicle; Mass spectrometry; Proteomics; PROLINE-RICH PROTEINS; FORMED IN-VIVO; SURFACE-PROPERTIES; PROTEOMIC ANALYSIS; HUMAN SALIVARY; HYDROXYAPATITE; IDENTIFICATION; STATHERIN; ADSORPTION; COMPONENTS;
D O I：
10.1159/000346280
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
We evaluated, by proteomic analysis, whether the chemical changes provoked on enamel by acidulated phosphate fluoride (APF) application alter the protein composition of acquired enamel pellicle. Enamel slabs, pretreated with distilled water (negative control), phosphoric acid (active control) or APF solution, were immersed in human saliva for pellicle formation. The adsorbed proteins were extracted and analyzed by liquid chromatography-mass spectrometry/mass spectrometry. Fifty-six proteins were identified, 12 exclusive to APF and 11 to phosphoric acid. APF decreased the concentration of histatin-1, but increased the concentration of S100-A9, which is confirmed by immunoblotting. The findings suggest that APF application changes the acquired enamel pellicle composition. Copyright (C) 2013 S. Karger AG, Basel
引用
收藏
页码：251 / 258
页数：8
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