The role of C1QBP in CSF-1-dependent PKCζ activation and macrophage migration

被引：10
作者：
Wang, Yong [1 ]
Su, Jing [2 ]
Yuan, Bo [2 ]
Fu, Donghe [2 ]
Niu, Yuanjie [1 ]
Yue, Dan [2 ]
机构：
[1] Tianjin Med Univ, Tianjin Inst Urol, Tianjin Med Univ Hosp 2, Dept Urol, Tianjin 300211, Peoples R China
[2] Tianjin Med Univ, Sch Lab Med, Tianjin 300203, Peoples R China
来源：
EXPERIMENTAL CELL RESEARCH | 2018年 / 362卷 / 01期
基金：
中国国家自然科学基金;
关键词：
C1QBP; CSF-1; Migration; Macrophage; PKC zeta; COLONY-STIMULATING FACTOR; RENAL-CELL CARCINOMA; TUMOR-ASSOCIATED MACROPHAGES; BINDING-PROTEIN; GLOBULAR HEADS; INTEGRIN TRAFFICKING; EXPRESSION; RECEPTOR; CANCER; CSF-1;
D O I：
10.1016/j.yexcr.2017.09.038
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Macrophages view as double agents in tumor progression. Trafficking of macrophages to the proximity of tumors is mediated by colony-stimulating factor-1 (CSF-1), a growth factor. In this study, we investigated the role of complement1q-binding protein (C1QBP)/ atypical protein kinase C zeta (PKC zeta) in CSF-1-induced macrophage migration. Disruption of C1QBP expression impaired chemotaxis and adhesion of macrophage. Phosphorylation of PKC zeta is an essential component in macrophage chemotaxis signaling pathway. C1QBP could interact with PKC zeta in macrophage. C1QBP knockdown inhibited CSF-1 induced phosphorylation of PKC zeta and integrin-beta 1. However, C1QBP knockdown didn't affect the phosphorylation of PKC zeta induced by MCP-1. Furthermore, CSF-1 from RCC cell condition medium promoted macrophage chemotaxis and adhesion. Taken together, our results demonstrated that C1QBP plays an essential role in CSF-1 induced migration of macrophages.
引用
收藏
页码：11 / 16
页数：6
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