Protein Glycosylation and Its Impact on Biotechnology

被引：34

作者：

Berger, Markus ^{[1
]}

Kaup, Matthias ^{[1
]}

Blanchard, Veronique ^{[1
]}

机构：

[1] Charite, Cent Inst Lab Med & Pathobiochem, D-10117 Berlin, Germany

来源：

GENOMICS AND SYSTEMS BIOLOGY OF MAMMALIAN CELL CULTURE | 2012年 / 127卷

关键词：

Biopharmaceuticals; Glycan analysis; Glycodesign; Glycoengineering; Glycosylation; HAMSTER OVARY CELLS; RECOMBINANT-HUMAN-ERYTHROPOIETIN; DEPENDENT CELLULAR CYTOTOXICITY; LINKED CARBOHYDRATE CHAINS; N-ACETYLNEURAMINIC ACID; IN-VIVO; PLASMINOGEN-ACTIVATOR; CHO-CELLS; MONOCLONAL-ANTIBODY; MASS-SPECTROMETRY;

D O I：

10.1007/10_2011_101

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glycosylation is a post-translational modification that is of paramount importance in the production of recombinant pharmaceuticals as most recombinantly produced therapeutics are N- and/or O-glycosylated. Being a cell-system-dependent process, it also varies with expression systems and growth conditions, which result in glycan microheterogeneity and macroheterogeneity. Glycans have an effect on drug stability, serum half-life, and immunogenicity; it is therefore important to analyze and optimize the glycan decoration of pharmaceuticals. This review summarizes the aspects of protein glycosylation that are of interest to biotechnologists, namely, biosynthesis and biological relevance, as well as the tools to optimize and to analyze protein glycosylation.

引用

页码：165 / 185

页数：21

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