Inactivation of Mycobacterium tuberculosis for DNA typing analysis

被引:34
作者
Bemer-Melchior, P [1 ]
Drugeon, HB [1 ]
机构
[1] Ctr Hospitalier Universitaire, Dept Microbiol, Nantes, France
关键词
D O I
10.1128/JCM.37.7.2350-2351.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
DNA fingerprinting analysis of Mycobacterium tuberculosis is used for epidemiological studies and the control of laboratory cross-contamination, Because standardized procedures are not entirely; safe for mycobacteriology laboratory staff, the paper proposes a new technique for the processing of specimens. The technique ensures the inactivation of M. tuberculosis before DNA extraction without the loss of DNA integrity. The control of inactivated cultures should be rigorous and should involve the use of two different culture media incubated for at least 4 months.
引用
收藏
页码:2350 / 2351
页数:2
相关论文
共 7 条
[1]   Results from 5 years of nationwide DNA fingerprinting of Mycobacterium tuberculosis complex isolates in a country with a low incidence of M-tuberculosis infection [J].
Bauer, J ;
Yang, ZH ;
Poulsen, S ;
Andersen, ÅB .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (01) :305-308
[2]  
BEMERMELCHIOR P, 1998, 5 INT C PREV INF, P113
[3]   Retrospective detection of laboratory cross-contamination of Mycobacterium tuberculosis cultures with use of DNA fingerprint analysis [J].
Braden, CR ;
Templeton, GL ;
Stead, WW ;
Bates, JH ;
Cave, MD ;
Valway, SE .
CLINICAL INFECTIOUS DISEASES, 1997, 24 (01) :35-40
[4]   MOLECULAR STRAIN TYPING OF MYCOBACTERIUM-TUBERCULOSIS TO CONFIRM CROSS-CONTAMINATION IN THE MYCOBACTERIOLOGY LABORATORY AND MODIFICATION OF PROCEDURES TO MINIMIZE OCCURRENCE OF FALSE-POSITIVE CULTURES [J].
SMALL, PM ;
MCCLENNY, NB ;
SINGH, SP ;
SCHOOLNIK, GK ;
TOMPKINS, LS ;
MICKELSEN, PA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (07) :1677-1682
[5]   STRAIN IDENTIFICATION OF MYCOBACTERIUM-TUBERCULOSIS BY DNA FINGERPRINTING - RECOMMENDATIONS FOR A STANDARDIZED METHODOLOGY [J].
VANEMBDEN, JDA ;
CAVE, MD ;
CRAWFORD, JT ;
DALE, JW ;
EISENACH, KD ;
GICQUEL, B ;
HERMANS, P ;
MARTIN, C ;
MCADAM, R ;
SHINNICK, TM ;
SMALL, PM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (02) :406-409
[6]   OCCURRENCE AND STABILITY OF INSERTION SEQUENCES IN MYCOBACTERIUM-TUBERCULOSIS COMPLEX STRAINS - EVALUATION OF AN INSERTION SEQUENCE-DEPENDENT DNA POLYMORPHISM AS A TOOL IN THE EPIDEMIOLOGY OF TUBERCULOSIS [J].
VANSOOLINGEN, D ;
HERMANS, PWM ;
DEHAAS, PEW ;
SOLL, DR ;
VANEMBDEN, JDA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (11) :2578-2586
[7]   RENDERING OF MYCOBACTERIA SAFE FOR MOLECULAR DIAGNOSTIC STUDIES AND DEVELOPMENT OF A LYSIS METHOD FOR STRAND DISPLACEMENT AMPLIFICATION AND PCR [J].
ZWADYK, P ;
DOWN, JA ;
MYERS, N ;
DEY, MS .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (09) :2140-2146