A contribution to the necessity for double testing of meat juice samples from pigs in ELISA to assess the salmonella incidence in piggeries

被引：0

作者：

Steinbach, G ^{[1
]}

Bahn, P ^{[1
]}

Kreutzer, B ^{[1
]}

Protz, D ^{[1
]}

Staak, C ^{[1
]}

机构：

[1] Bundesinst Gesundheit Verbrauch & Vet Med, Fachbereich Bakt Tierzeuchen & Bekampf Zoo 4, D-07743 Jena, Germany

来源：

BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT | 1999年 / 112卷 / 04期

关键词：

salmonella antibodies; meat juice; serum; pigs; ELISA; double testing;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

According to the test protocol of the "meat juice ELISA"" for detection of salmonella antibodies in pigs, all meat juice samples and serum controls an to be tested in duplicate. Results from routine investigations of repeatedly double tested meat juice and serum samples have been used to analyze the effect of double testing versus single testing with regard to the reliability of the final result. In case of an individual animal. testing of samples in duplicate increases the reliability of the results significantly, especially, if samples are retested at different occasions. In contrast, such a difference between mono and double testing of samples is not of importance when a group of animals is tested in order to determine the mean antibody rate in a herd. Here, results from double testing practically do not contribute to a higher reliability of the final result. This observation provides the possibility to reduce the costs for investigation programmes drastically.

引用

页码：121 / 123

页数：3