Production, Purification and Characterization of Siderophore from Pseudomonas fluorescence

In the present study plant growth promoting rhizobacterial strains belonging to fluorescent pseudomonads were isolated from rhizosphere soil and screened for siderophore production. Among these isolates, the potent strain was identified by biochemical and 16S ribosomal DNA gene sequencing and the isolate was confirmed as Pseudomonas fluorescence. Optimized production of siderophore was carried out utilizing various sources such as carbon, nitrogen, organic acids, amino acids and pH (5-9). Siderophore production was optimum during 24-30 h incubatory span at pH 7, 29 degrees C temperature. Other parameters investigated were sugars, nitrogen sources, organic acids, amino acids, metal ions and iron (FeCl3). The siderophore was purified using Amber lite XAD-4 column chromatographic technique. The purified siderophore was characterized by FTIR spectral analysis.