Genetic diversity and population differentiation of the Chinese soft-shelled turtle (Pelodiscus sinensis) in three geographical populations

被引：14

作者：

Bu, Xingjiang ^{[1
]}

Liu, Luo ^{[1
]}

Nie, Liuwang ^{[1
]}

机构：

[1] Anhui Normal Univ, Life Sci Coll, Prov Key Lab Conservat & Exploitat Res Biol Resou, Wuhu 241000, Peoples R China

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2014年 / 54卷

基金：

国家教育部博士点专项基金资助; 中国国家自然科学基金;

关键词：

Pelodiscus sinensis; Microsatellite; Genetic diversity; Population differentiation; MULTIPLE PATERNITY; MICROSATELLITE LOCI; FREQUENCY;

D O I：

10.1016/j.bse.2014.02.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Chinese soft-shelled turtle (Pelodiscus sinensis) is one of the most important economical chelonians in the world. To understand the genetic variations of the Chinese soft-shelled turtle in China, 62 individuals were sampled from three localities and 18 polymorphic microsatellite loci tested were used to detect genetic diversity and population structure. Results showed that the genetic diversity of the wild P. sinensis was high. Except for the Wuhu populations, the majority of microsatellite loci are not deviation from Hardy-Weinberg equilibrium in the other two populations. AMOVA analysis indicated that genetic variations occurred mainly within populations (97.4%) rather than among populations (2.6%). The gene flow estimates (Nm) among three geographic populations demonstrated that strong gene flow existed (Nm > 1, mean 6). The present study supported that different habitats, breed turtles escaped, multiple paternity and long evolutionary history may be responsible for the current genetic diversity and differentiation in the wild Chinese soft-shelled turtle. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：279 / 284

页数：6

共 25 条

[1] Genetic diversity, social structure, and conservation value of the elephants of the Nakai Plateau, Lao PDR, based on non-invasive sampling [J].

Ahlering, Marissa A. ;

Hedges, Simon ;

Johnson, Arlyne ;

Tyson, Martin ;

Schuttler, Stephanie G. ;

Eggert, Lori S. .

CONSERVATION GENETICS, 2011, 12 (02) :413-422

[2]

Altherr Sandra, 2000, Turtle and Tortoise Newsletter, V1, P7

[3]

[Anonymous], 2013, TAXONOMY CHINESE CHE

[4]

[Anonymous], 2012, Molecular Cloning: A Laboratory Manual

[5] Genetic diversity and structure of blue whales (Balaenoptera musculus) in Australian feeding aggregations [J].

Attard, Catherine R. M. ;

Beheregaray, Luciano B. ;

Jenner, Curt ;

Gill, Peter ;

Jenner, Micheline ;

Morrice, Margaret ;

Bannister, John ;

LeDuc, Rick ;

Moeller, Luciana .

CONSERVATION GENETICS, 2010, 11 (06) :2437-2441

[6] Isolation and characterization of 21 novel polymorphic microsatellite loci in the Chinese soft-shelled turtle Pelodiscus sinensis [J].

Bu, X. J. ;

Liu, L. ;

Wang, L. ;

Nie, L. W. .

GENETICS AND MOLECULAR RESEARCH, 2011, 10 (02) :1006-1010

[7] The effects of incubation thermal environments on size, locomotor performance and early growth of hatchling soft-shelled turtles, Pelodiscus sinensis [J].

Do, WG ;

Ji, X .

JOURNAL OF THERMAL BIOLOGY, 2003, 28 (04) :279-286

[8] Arlequin (version 3.0): An integrated software package for population genetics data analysis [J].

Excoffier, Laurent ;

Laval, Guillaume ;

Schneider, Stefan .

EVOLUTIONARY BIOINFORMATICS, 2005, 1 :47-50

[9]

Lara-De La Cruz LI, 2010, HIDROBIOLOGICA, V20, P85

[10] Detection of multiple paternity and sperm storage in a captive colony of the central Asian tortoise, Testudo horsfieldii [J].

Johnston, Emily E. ;

Rand, Matthew S. ;

Zweifel, Stephan G. .

CANADIAN JOURNAL OF ZOOLOGY, 2006, 84 (04) :520-526

← 1 2 3 →