Optimization of Enzymatic Extraction of Peanut Protein by Response Surface Methodology

被引：0

作者：

Lv, Yan Hong ^{[1
]}

Zhang, Shao Bing ^{[2
]}

Tang, Zhou ^{[2
]}

机构：

[1] Henan Univ Technol, Sch Biol Engn, Zhengzhou 450052, Henna, Peoples R China

[2] Henan Univ Technol, Sch Food Sci & Technol, Zhengzhou 450052, Henna, Peoples R China

来源：

ISBE 2011: 2011 INTERNATIONAL CONFERENCE ON BIOMEDICINE AND ENGINEERING, VOL 3 | 2011年

关键词：

enzymatic extraction; protease; peanut protein; response surface methodology;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Four kinds of proteases including Alcalase 2.4L,2709 alkline protease, AS1398 neutral protease and Protex 6L were used to extract peanut protein, respectively. 2709 alkline protease showed the highest extraction efficacy, followed by Protex 6L. Response surface methodology (RSM) was used to study the effects of 2709 alkline protease on extraction of peanut protein. The highest protein yield was 94.3% under the optimal conditions (solid-to-water ratio 1:4, alkaline pH 9.0, enzyme amount 3.75g (in 60g of peanut seeds) and hydrolysis time 1.5 h. By analysis of the experimental results, a suitable model was obtained for predicting the yield of protein (R-2 = 0.8182, p<0.01). The solid-to-water ratio highly significantly (p<0.01) affected the protein yield.

引用

页码：342 / 346

页数：5

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