Characterization of an alanine racemase gene from Lactobacillus reuteri

被引：7
|
作者：
Thompson, A [1 ]
Griffin, H [1 ]
Gasson, MJ [1 ]
机构：
[1] Food Res Inst, Norwich NR4 7UA, Norfolk, England
来源：
CURRENT MICROBIOLOGY | 2002年 / 44卷 / 04期
关键词：
D O I：
10.1007/s00284-001-0030-8
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
An alanine racemase gene from Lb. reuteri was cloned by using degenerate oligonucleotides corresponding to conserved regions derived from several bacterial alanine racemases. The protein is 375alphaalpha in length and shows 63.6% homology to the Lb. plantarum alanine racemase. Unlike the single alanine racemase activity found in Lb. plantarum, deletion of the Lb. reuteri alanine racemase reveals a second activity, which is inhibited by P-chloro-D-alanine.
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页码：246 / 250
页数：5
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