Functions of corneal endothelial cells do not change after uptake of superparamagnetic iron oxide nanoparticles

被引:11
|
作者
Bi, Yan-Long [1 ]
Wu, Ming-Feng [1 ]
Lu, Li-Xia [2 ,3 ]
Zhou, Qi [1 ]
Du, Fei [1 ]
Sun, Xiao-Ting [1 ]
Tang, Sen-Fei [1 ]
Xu, Guo-Tong [2 ,3 ]
机构
[1] Tongji Univ, Tongji Hosp, Sch Med, Dept Ophthalmol, Shanghai 200065, Peoples R China
[2] Tongji Univ, Sch Med, Dept Regenerat Med, Shanghai 200092, Peoples R China
[3] Tongji Univ, Sch Med, Stem Cell Res Ctr, Shanghai 200092, Peoples R China
关键词
corneal endothelial cell; superparamagnetic iron oxide nanoparticles; cell cycle progression; Na+/K+-ATPase; short circuit current; IN-VITRO; PUMP FUNCTION; TRANSPLANTATION; JUNCTIONS; SURVIVAL; MODEL; VIVO;
D O I
10.3892/mmr.2013.1418
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
To avoid donor tissue shortages, ex vivo cultured human corneal endothelial cell (HCEC) transplantation is a promising therapeutic resource. Superparamagnetic iron oxide nanoparticle (SPION) cell labeling assists HCEC transplantation by attaching the posterior corneal stroma in ex vivo animal models. However, possible functional changes of the HCECs following SPION labeling remain to be determined. In this study, we used SPIONs to label cultured rabbit CECs (RCECs) in order to observe important cell functions and the levels of cell markers. The synthetic SPIONs exhibited superparamagnetism at room temperature, with saturation magnetization of 55.4 emu/g and negligible remanence or coercivity. The zeta-potential was -24.5 mV and the diameter was 101 +/- 55 nm. Immunostaining demonstrated a normal density of zonula occluden-1 (ZO-1), nestin and Ki-67 at cellular junctions or in nuclei from RCECs following SPION labeling at 16 mu g/ml. MTT cytotoxicity assay, homotypic adhesion assay, quantitative flow cytometric Ki-67 analysis and RCEC pump function measurement demonstrated no significant differences between the cells with or without SPION labeling (P<0.05, for all assays). Results of this study demonstrated successful labeled cultured RCECs with synthetic SPIONs. Labeled cells possessed several important characteristics required to maintain the transparency and refractive parameters of the cornea, including hexagonal cell morphology, higher cell adhesion ability and proliferative potential, cell pump function and the positive expression of several cell markers.
引用
收藏
页码:1767 / 1772
页数:6
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