Dry powder preparation of inulin fructotransferase from Arthrobacter aurescens SK 8.001 fermented liquor

被引：9

作者：

Hang, Hua ^{[1
,2
]}

Li, Yungao ^{[1
]}

Zhao, Meng ^{[3
]}

Jiang, Bo ^{[1
]}

Miao, Ming ^{[1
]}

Mu, Wanmeng ^{[1
]}

Zhang, Tao ^{[1
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Anhui Normal Univ, Coll Environm Sci & Engn, Wuhu 241000, Peoples R China

[3] Hubei Univ Technol, Glyn O Phillips Hydrocolloids Res Ctr, Wuhan 430068, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2013年 / 95卷 / 02期

关键词：

DFA III; IFTase; Ultrafiltration; Ammonium sulfate precipitation; Freeze drying; DIFRUCTOSE ANHYDRIDE-III; CALCIUM-ABSORPTION; PURIFICATION;

D O I：

10.1016/j.carbpol.2013.02.058

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Difructosan anhydrides III (DFA III) are usually obtained by inulin conversion with inulin fructotransferase (IFTase). IFTase liquor is difficult to store for a long time, which could greatly restrict its application and DFA III production. To meet DFA III scale-up preparation, this work was explored to research dry powder preparation of IFTase from Arthrobacter aurescens SK 8.001 fermented liquor by ultrafiltration concentration, ammonium sulfate precipitation and freeze drying. IFTase powder (10.2g) was obtained from IFTase precipitation (126.4g) and its specific activity determined was 16.4 U/mg. Dry powder of IFTase could maintain over 120 days at different temperatures. These results showed that it is easy to scale up DFA III preparation for industrial capacity. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：654 / 656

页数：3

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