Visualization of proteins in acrylamide gels using ultraviolet illumination

被引：46

作者：

Kazmin, D ^{[1
]}

Edwards, RA

Turner, RJ

Larson, E

Starkey, J

机构：

[1] Montana State Univ, Dept Chem & Biochem, Bozeman, MT 59717 USA

[2] Montana State Univ, Dept Microbiol, Bozeman, MT 59717 USA

[3] Univ Calgary, Dept Biol Sci, Struct Biol Res Grp, Calgary, AB T2N 1N4, Canada

来源：

ANALYTICAL BIOCHEMISTRY | 2002年 / 301卷 / 01期

关键词：

SDS-PAGE; protein detection; ultraviolet; staining; tryptophan; tryptophan photomodification; fluorescence;

D O I：

10.1006/abio.2001.5488

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins in polyacrylamide gels can be rapidly visualized by soaking in trichloroacetic acid or chloroform followed by UV reaction of tryptophan in the presence of trichlorocompounds yields products that emit sufficiently in the visible region to identify the location of the protein bands on the gel. This method can be used to rapidly identify protein bands on a gel in less than 20 min. On thin polyacrylamide gels, 1.0 mug of protein can easily be detected for proteins with typical tryptophan percentages. (C) 2002 Elsevier Science.

引用

页码：91 / 96

页数：6

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