Species identification of aphids (Insecta: Hemiptera: Aphididae) through DNA barcodes

被引：234
作者：
Foottit, R. G. [1 ]
Maw, H. E. L. [1 ]
Von Dohlen, C. D. [2 ]
Hebert, P. D. N. [3 ]
机构：
[1] Agr & Agri Food Canada, Invertebrate Biodivers, Natl Environm Hlth Program, Ottawa, ON K1A 0C6, Canada
[2] Utah State Univ, Dept Biol, Logan, UT 84322 USA
[3] Univ Guelph, Dept Integrat Biol, Biodivers Inst Ontario, Guelph, ON N1G 2W1, Canada
来源：
MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 06期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Aphididae; COI; DNA barcoding; mitochondrial DNA; parthenogenesis; species identification;
D O I：
10.1111/j.1755-0998.2008.02297.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A 658-bp fragment of mitochondrial DNA from the 5 ' region of the mitochondrial cytochrome c oxidase 1 (COI) gene has been adopted as the standard DNA barcode region for animal life. In this study, we test its effectiveness in the discrimination of over 300 species of aphids from more than 130 genera. Most (96%) species were well differentiated, and sequence variation within species was low, averaging just 0.2%. Despite the complex life cycles and parthenogenetic reproduction of aphids, DNA barcodes are an effective tool for identification.
引用
收藏
页码：1189 / 1201
页数：13
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