Gene transfer using human polyomavirus BK virus-like particles expressed in insect cells
被引:42
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作者:
Touzé, A
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机构:Fac Sci Pharmaceut Philippe Maupas, IFR Transposons & Virus, Virol Mol Lab, F-37200 Tours, France
Touzé, A
Bousarghin, L
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机构:Fac Sci Pharmaceut Philippe Maupas, IFR Transposons & Virus, Virol Mol Lab, F-37200 Tours, France
Bousarghin, L
Ster, C
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机构:Fac Sci Pharmaceut Philippe Maupas, IFR Transposons & Virus, Virol Mol Lab, F-37200 Tours, France
Ster, C
Combita, AL
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机构:Fac Sci Pharmaceut Philippe Maupas, IFR Transposons & Virus, Virol Mol Lab, F-37200 Tours, France
Combita, AL
Roingeard, P
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机构:Fac Sci Pharmaceut Philippe Maupas, IFR Transposons & Virus, Virol Mol Lab, F-37200 Tours, France
Roingeard, P
Coursaget, P
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机构:Fac Sci Pharmaceut Philippe Maupas, IFR Transposons & Virus, Virol Mol Lab, F-37200 Tours, France
Coursaget, P
机构:
[1] Fac Sci Pharmaceut Philippe Maupas, IFR Transposons & Virus, Virol Mol Lab, F-37200 Tours, France
[2] Fac Med, IFR Transposons & Virus, Labs Biol Cellulaire & Virol, F-37032 Tours, France
来源:
JOURNAL OF GENERAL VIROLOGY
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2001年
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82卷
关键词:
D O I:
10.1099/0022-1317-82-12-3005
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
The major structural protein (VP1) of the BK polyomavirus (BKV) was expressed in the recombinant baculovirus expression system. Recombinant BKV VP1 was shown to self-assemble into virus-like particles (VLPs) with a diameter of 45-50 rim. As for other polyomaviruses, BKV VP1 has the capacity to bind to exogenous DNA. Furthermore, the potential of BKV VP1 VLPs was investigated for gene transfer into COS-7 cells using three methods for the formation of pseudo-virions: disassembly/reassembly, osmotic shock and direct interaction between VLPs and reporter plasmid DNA. The latter method was shown to be the most efficient when using linearized plasmid. Gene transfer efficiency with BKV pseudo-virions was of the same order as that observed with human papillomavirus type 16 L1 protein VLPs. In addition, it is demonstrated that cellular entry of BKV pseudo-virions is dependent on cell surface sialic acid.