Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry

被引：14

作者：

Jumaah, Firas ^{[1
]}

Sandahl, Margareta ^{[1
]}

Turner, Charlotta ^{[1
]}

机构：

[1] Lund Univ, Dept Chem, Ctr Anal & Synth, S-22100 Lund, Sweden

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 2015年 / 92卷 / 08期

基金：

瑞典研究理事会;

关键词：

Bilberry; Supercritical fluid extraction; Supercritical fluid chromatography; GC-MS; Lipids; Fatty acids; ANTHOCYANINS; PROFILES; FATS;

D O I：

10.1007/s11746-015-2680-x

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 A degrees C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAME) was found to be 4.84 +/- A 0.06 mg and 4.564 +/- A 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 +/- A 6.06 mg and 65.70 +/- A 0.67 mg per g of sample for SFE and B&D, respectively. This discrepancy between FAME and total lipids could be explained by the presence of wax esters, sterol esters, carotenoids and phospholipids, as determined by supercritical fluid chromatography.

引用

页码：1103 / 1111

页数：9

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