RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF METRONIDAZOLE AND OFLOXACIN IN SYNTHETIC MIXTURE

被引:3
作者
Maslarska, Vania [1 ]
Tsvetkova, Boyka [1 ]
Peikova, Lily [1 ]
Bozhanov, Stanislav [1 ]
机构
[1] Med Univ Sofia, Fac Pharm, Dept Chem, 2 Dunav St, Sofia 1000, Bulgaria
来源
CBU INTERNATIONAL CONFERENCE PROCEEDINGS 2016: INNOVATIONS IN SCIENCE AND EDUCATION | 2016年 / 4卷
关键词
metronidazole; ofloxacin; RP-HPLC; validation; drugs; quality control;
D O I
10.12955/cbup.v4.871
中图分类号
F [经济];
学科分类号
02 ;
摘要
Development and validation of a high-performance liquid chromatographic analytical procedure for simultaneously determining metronidazole and ofloxacin in a synthetic mixture is described in this paper. The separation was made with a LiChrosorb r RP-18 (250 x 4.6 mm) column, at 30 degrees C temperature, with isocratic mode and mobile phase, containing triethylamine, acetonitrile, and 0.3% o-phosphoric acid solution (0.02: 20: 80 v/v/v). Eluent was monitored at 290 nm and the flow rate was 1.0 ml/min. Metronidazole and ofloxacin were effectively separated with retention time (t(r)) of 3.42 min and 6.15 min, respectively, within the selected chromatographic conditions. The method was validated for analytical parameters: specificity, linearity, precision, accuracy, and limits of detection and quantitation. The calibration curves were linear in the concentration range of 12.5 to 100.0 mu g/ml for metronidazole and ofloxacin, and the regression coefficients were more than 0.999. For metronidazole and ofloxacin the recovery was 100.01% and 100.04%, respectively. This analytical procedure is applicable for the quality control of drug formulations.
引用
收藏
页码:900 / 905
页数:6
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