Anxiolytic- and antidepressant-like actions of Urocortin 2 and its fragments in mice

被引:5
作者
Bagosi, Zsolt [1 ]
Csabafi, Krisztina [1 ]
Balango, Beata [1 ]
Pinter, David [1 ]
Szolomajer-Csikos, Orsolya [2 ]
Bozso, Zsolt [2 ]
Toth, Gabor [2 ]
Telegdy, Gyula [1 ]
Szabo, Gyula [1 ]
机构
[1] Univ Szeged, Fac Med, Dept Pathophysiol, Szeged, Hungary
[2] Univ Szeged, Fac Med, Dept Med Chem, Szeged, Hungary
关键词
Urocortin; 2; fragments; Anxiolytic action; Antidepressant action; C57BL/6; mice; CORTICOTROPIN-RELEASING-FACTOR; FACTOR-BINDING-PROTEIN; ANXIETY-LIKE BEHAVIOR; FORCED SWIMMING TEST; FISH UROTENSIN-I; FACTOR CRF; HORMONE-RECEPTOR; STRESS-RESPONSE; PITUITARY; PEPTIDE;
D O I
10.1016/j.brainres.2017.12.011
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The aim of the present study was to investigate the potential anxiolytic- and antidepressant-like actions of Urocortin 2 (Ucn2) and its two fragments, Ucn2 (1-21) and Ucn2 (22-38), in mice, in an attempt to identify the biologically active sequence of this 38 amino acid neuropeptide. In this purpose, male C57BL/6 mice were treated intracerebroventricularly (icv) with 0.125, 0.25, 0.5 and 1 mu g/2 mu l of Ucn2, Ucn2 (1-21) or Ucn2 (22-38). After 30 min, the mice were evaluated in an elevated plus-maze test and a forced swim test for anxiety- and depression-like behavior, respectively. Each test lasted 5 min. Ucn2 at dose of 0.25 mu g/2 mu l and Ucn2 (1-21) at dose of 0.125 mu g/2 mu l, but not Ucn2 (22-38), increased significantly the number of entries into and the time spent in the open-arms, without influencing the total number of entries. In parallel, the same doses of Ucn2 and Ucn2 (1-21), but not Ucn2 (22-38), increased significantly the climbing and the swimming activity, while decreasing significantly the time of immobility. In addition, Ucn2 at doses of 0.125 mu g/2 mu l and 0.5 mu g/2 mu l decreased significantly the time of immobility, but they did not change the other parameters. The present study demonstrates that Ucn2 exerts anxiolytic- and antidepressant-like effects in C57BL/6 mice, which are mediated by the N-terminal, but not the C-terminal fragment of the peptide. The establishment of the smallest active sequence by further fragmentation of Ucn2 (1-21) may allow the synthesis of new anxiolytic and antidepressant drugs. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:62 / 68
页数:7
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