Infrared surface plasmon spectroscopy and biosensing

被引：0

作者：

Yashunsky, Victor ^{[1
]}

Zilbershtein, Alexander ^{[1
]}

Lirtsman, Vladislav ^{[1
]}

Marciano, Tal ^{[1
]}

Aroeti, Benjamin ^{[2
]}

Golosovsky, Michael ^{[1
]}

Davidov, Dan ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Racah Inst Phys, IL-91904 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Dept Dev & Cell Biol, IL-91904 Jerusalem, Israel

来源：

PLASMONICS IN BIOLOGY AND MEDICINE IX | 2012年 / 8234卷

关键词：

Living Cells; Biosensor; Surface Plasmon; Label-free; Noninvasive method; Evanescent Waves; Infrared spectroscopy; RESONANCE; CELLS;

D O I：

10.1117/12.907255

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The cell morphology is a valuable indicator of the physical condition and general status of the cell. Here we demonstrate a methodology for noninvasive biosensing of adherent living cells. Our method is based on infrared reflection spectroscopy of living cells cultured on thin Au film. To characterize cell morphology we utilized the unique properties of the infrared surface plasmon (lambda = 1-3 mu m) and infrared guided wave that travel inside the cell monolayer. We demonstrate that our method enables monitoring of submicron variations in cell morphology in real-time and in a label-free manner. In addition to morphological characterization, our method allows investigation of chemical composition and molecular structure of cells through infrared absorption spectroscopy analysis.

引用

页数：7

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